TY - JOUR T1 - LATE-BREAKING ABSTRACT: Do mesenchymal stromal cells provide an organ-specific tissue niche? – A proteomic matrisome characterization JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2016.OA503 VL - 48 IS - suppl 60 SP - OA503 AU - Sara Rolandsson Enes AU - Emma Åhrman AU - Anitha Palani AU - Oskar Hallgren AU - Leif Bjermer AU - Stefan Scheding AU - Anders Malmström AU - Johan Malmström AU - Gunilla Westergren-Thorsson Y1 - 2016/09/01 UR - http://erj.ersjournals.com/content/48/suppl_60/OA503.abstract N2 - Background: The mesenchymal stromal cells (MSC) are potent candidates for cell therapies, due to their immune-regulatory and regenerative properties. In most clinical trials bone marrow-derived MSC have been used, also for treatment of various pulmonary diseases. Bone marrow-derived MSC play an important role within the bone marrow, by providing and maintaining a functioning niche for the hematopoiesis. The functional role of lung-resident MSC, on the other hand, is still unknown. Exploring the proteins produced by lung-derived MSC might improve our understanding of the functional role of MSC within the lung. Therefore, we aimed to characterize the proteins produced by lung-derived MSC and to compare those findings to the proteins produced by MSC isolated from bone marrow.Methods: Proteins within the cell-layer and the conditioned medium of MSC isolated from lung biopsies and bone marrow aspirates were characterized using mass spectrometry-based quantitative proteomics.Results: Our results indicate that MSC isolated from lung biopsies and bone marrow aspirates are prominent producers of extracellular matrix proteins. Furthermore, these analyses revealed differences between lung- and bone marrow-derived MSC, both regarding proteins identified in the cell layer and within the conditioned medium.Conclusions: Lung-derived MSC are prominent extracellular matrix producers, and our results indicate that lung- and bone marrow-derived MSC produce different protein profiles. ER -