PT - JOURNAL ARTICLE AU - Masaru Suzuki AU - Satoshi Konno AU - Hironi Makita AU - Kaoruko Shimizu AU - Hiroki Kimura AU - Hirokazu Kimura AU - Masaharu Nishimura TI - Altered circulating exosomal RNA profiles detected by next-generation sequencing in patients with severe asthma AID - 10.1183/13993003.congress-2016.PA3410 DP - 2016 Sep 01 TA - European Respiratory Journal PG - PA3410 VI - 48 IP - suppl 60 4099 - http://erj.ersjournals.com/content/48/suppl_60/PA3410.short 4100 - http://erj.ersjournals.com/content/48/suppl_60/PA3410.full SO - Eur Respir J2016 Sep 01; 48 AB - Introduction: Exosomes are 20-100 nm vesicles secreted by various cell types and contain RNA population that closely reflects the biologic state of parent cells. Therefore, circulating exosomal RNA could be a potential biomarker for a variety of diseases. However, little is known about circulating exosomal RNA profiles in patients with asthma.Aim: To investigate the difference of serum exosomal RNA profiles among patients with mild-to-moderate or severe asthma and healthy volunteers.Methods: Serum exosomal RNA was extracted from 12 subjects (4 mild-to-moderate and 4 severe asthma, and 4 healthy volunteers), and next-generation sequencing on the Illumina platform was performed by the Exosome RNA Next-Gen Sequencing Service (System Biosciences, USA).Results: Relative abundances of various RNA types were significantly altered in patients with severe asthma with a decrease in antisense to repeat elements and increases in antisense to exons and unannotated RNA. Moreover, principal component analysis and volcano plots revealed that differential expression profiles of specific RNA sequences apparently differed in patients with severe asthma compared with other groups. MicroRNA analysis revealed that miR-128, miR-140-3p, miR-196b-5p, and miR-486-5p were significantly up-regulated in patients with severe asthma (p<0.003, FDR q<0.20), and DIANA-mirPath analysis identified ErbB signaling pathway, focal adhesion, and Neurotrophin signaling pathway as top-ranked KEGG pathways regulated by the up-regulated microRNAs.Conclusion: The profiles of serum exosomal RNA were altered in patients with severe asthma compared with those with mild-to-moderate asthma and healthy volunteers.