RT Journal Article SR Electronic T1 Bronchial epithelial gene expression of NOX isoforms are related to important clinical characteristics in COPD JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP PA4012 DO 10.1183/13993003.congress-2016.PA4012 VO 48 IS suppl 60 A1 Richard Russell A1 Fay Hollins A1 María Soler Artigas A1 Anna Esteve-Codino A1 Gian Andri Thun A1 Chris Newby A1 Louise V. Wain A1 Martin D. Tobin A1 Simon Heath A1 Ivo Gut A1 Deepak Subramanian A1 Sumit Gupta A1 David Parr A1 Dave Singh A1 Loems Ziegler-Heitbrock A1 Chris Brightling YR 2016 UL http://erj.ersjournals.com/content/48/suppl_60/PA4012.abstract AB Chronic Obstructive Pulmonary Disease (COPD) is characterised by persistent airflow obstruction, airway inflammation and increased oxidative stress. The Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidases (NOX1-5 and DUOX1-2) are implicated in the generation of reactive oxygen species, but their role in COPD is uncertain.As part of the EvA study bronchial brushings were obtained from 279 COPD subjects and 215 healthy controls. Gene expression of the NOX isoforms was analysed; compared between COPD and health and multiple regression analyses of NOX expression and clinical, physiological, and quantitative (q)CT parameters were performed in COPD cases.Gene expression of NOX2, 4 and DUOX2 was increased significantly and NOX1 decreased in COPD compared to controls, whereas NOX5 and DUOX1 expression was not different between groups and NOX3 was not present. Multiple regression analyses identified independent associations between: NOX1 and DUOX2 with airflow obstruction (FEV1/FVC ratio) (β=0.006[0.003]; P=0.046 and β=-0.016[0.008]; P=0.035 respectively); NOX1 and NOX2 with airway inflammation (NOX1 with sputum neutrophil and eosinophil % β=0.004[0.002]; P=0.016 and β=-0.056[0.028]; P=0.047 and NOX2 with sputum eosinophils % β=0.004[0.002]; P=0.016); and NOX4 with body mass index (β=0.022[0.005]; P<0.001). NOX isoforms were not independently related to FEV1% predicted or qCT parameters.In conclusion, NOX1, 2 and 4, and DUOX2 are differentially expressed between COPD and health. Associations between these NOX isoforms and important clinical characteristics suggest a potential role for NOX in the pathogenesis of COPD.