PT  - JOURNAL ARTICLE
AU  - Yanqiu Wu
AU  - Yongchun Shen
AU  - Xue Zhang
AU  - Hao Wang
AU  - Fuqiang Wen
TI  - Cryptotanshinone attenuates airway Inflammation induced by cigarette smoke in mice
AID  - 10.1183/13993003.congress-2016.PA4021
DP  - 2016 Sep 01
TA  - European Respiratory Journal
PG  - PA4021
VI  - 48
IP  - suppl 60
4099  - http://erj.ersjournals.com/content/48/suppl_60/PA4021.short
4100  - http://erj.ersjournals.com/content/48/suppl_60/PA4021.full
SO  - Eur Respir J2016 Sep 01; 48
AB  - Backgrounds and aims: Cigarette smoke (CS) is known to cause airway inflammation. Cryptotanshinone(CTS), a major ingredients of tanshinones, has been reported to have anti-inflammatory, antioxidative and other therapeutical effects. We designed this study to investigate the potential protective effects of CTS on CS-induced airway inflammation and oxidative stress in mice and the possible mechanisms.Methods: BALB/c mice were exposed to CS for 2.5 h twice daily, 6 days per week for 4 weeks. Low-dose (15 mg/kg·d) or high-dose (30 mg/kg·d) CTS were administered intraperitoneally 1 h before CS exposure. Bronchoalveolar lavage fluid (BALF) was acquired for detection of pro-inflammatory cytokine levels. Lung tissue was collected for histological examination, superoxide dismutase (SOD) activities, malondialdehyde (MDA) levels, and western blot assays for lectin-like oxidized low-density lipoprotein-1 receptor (LOX-1) and phosphorylated NF-κB p65.Results: CTS administration attenuated CS-induced thickening of the airway epithelium and peribronchial inflammatory cell infiltration. Moreover, CTS remarkably reduced the CS-induced elevation of TNF-α (12.99±3.37 vs 23.08±2.17 pg/ml), IL-17 (40.04±16.51 vs 124.84±7.04 pg/ml), and MCP-1 (33.85±7.76 vs 79.06±11.98 pg/ml) levels in BALF (all p<0.05). CTS pretreatment also prevented CS-induced elevation in MDA levels and decrease in SOD activities (p<0.05). Furthermore, the CS-induced expression of LOX-1 and the phosphorylation of p65 were also attenuated by CTS.Conclusions: These results suggest that CTS attenuated inflammation and oxidative stress induced by CS. The LOX-1 and NF-κB pathway may involve in this process.