RT Journal Article
SR Electronic
T1 Oral and nasal microbiota in healthy, tobacco smoke COPD (TSCOPD) and biomass smoke COPD (BMSCOPD) subjects from India
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3403
DO 10.1183/13993003.congress-2016.PA3403
VO 48
IS suppl 60
A1 Dhiraj Agarwal
A1 Dhiraj Dhotre
A1 Bill Brashier
A1 Yogesh Shouche
A1 Sanjay Juvekar
A1 Sundeep Salvi
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA3403.abstract
AB Background: Microbiota in the lower respiratory tract is largely derived from microaspirations from oral and nasal cavities and has been shown to vary between healthy and TSCOPD in the western world.Aim: To study and compare the microbiota from oral and nasal cavities of healthy, TSCOPD and BMSCOPD subjects from a rural population.Methods: Oral washing and nasal swab samples were collected from 12 healthy, 12 TSCOPD and 12 BMSCOPD subjects. Samples were sequenced using 16S rRNA gene v4 region (Illumina HiSeq, 2500 platform, USA). Downstream analysis was carried out using QIIME pipeline (v1.8), Mothur software (v1.34) and R scripts.Results: Significant differences were observed in bacterial diversity and their abundance between healthy and COPD subjects (p<0.05). Beta diversity analysis revealed differences in microbiota between BMSCOPD and TSCOPD subjects. At genus level, Neisseria, Prevotella, Streptococcus, Staphylococcus, Moraxella, Rothia and Haemophilus were found to differ in their abundance between study groups.Conclusion: Oral and nasal microbiota are significantly different between healthy and COPD subjects. Within the COPD group, BMSCOPD subjects had different microbiota than SMCOPD subjects. The clinical phenotypic differences observed between TSCOPD and BMSCOPD could be due to differences in microbial communities, which requires further research.