RT Journal Article
SR Electronic
T1 Role of the T-cell homing receptor in the immune mechanisms of the allergic march
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA1080
DO 10.1183/13993003.congress-2016.PA1080
VO 48
IS suppl 60
A1 Laure Castan
A1 Marie-Aude Cheminant
A1 Philippe Aubert
A1 Michel Neunlist
A1 Marie Bodinier
A1 Antoine Magnan
A1 Grégory Bouchaud
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA1080.abstract
AB Background: Allergic diseases are increasing for the past years. According to the World Health Organization, allergic diseases represent the fourth pathology in term of morbidity. The allergic march is characterized by the progression of the disease from food allergy during childhood to asthma and rhinitis during adulthood.Aims: The evolution in term of symptoms as well of inflammation suggests an implication of the T-cell homing receptors which are responsible for the immune cell migration toward inflamed tissues and organ. We are trying to find out how chemokine receptors orchestrate allergic evolution.Method: In a murine model of allergic march, combining a food allergy to wheat and a model of acute asthma to house dust mite, we analyzed the expression of chemokine receptors. Balb/c mice were intraperitoneally sensitized to wheat proteins and orally exposed to the same allergen. One week later, they were sensitized with house dust mite on the ear and challenged with intra-nasal exposure to induce asthma.Results: The results suggest an aggravation of the pulmonary symptoms in allergic march mice compared to asthmatic mice. These mice display a higher histological score and pulmonary resistance. Moreover, we observed an increase of neutrophils and lymphocytes CD4+CCR4+ in consecutively allergic mice. Finally, in vitro, we observed an increase of the migration of the lymphocytes from the lamina propria to the chemokine CCL25 in atopic march mice, suggesting a persistent role of CCR9.Conclusion: The induction of acute asthma after a food allergy protocol on mice leads to a worsening of pulmonary symptoms, suggesting a link between unrelated allergies involving a migratory process.