PT  - JOURNAL ARTICLE
AU  - Hoeke Baarsma
AU  - Wioletta Skronska-Wasek
AU  - Kathrin Mutze
AU  - Florian Ciolek
AU  - Gerrit John-Schuster
AU  - Ron Smits
AU  - Ali Önder Yildirim
AU  - Melanie Königshoff
TI  - Non-canonical WNT-5A signaling attenuates endogenous lung repair in COPD
AID  - 10.1183/13993003.congress-2016.PA4014
DP  - 2016 Sep 01
TA  - European Respiratory Journal
PG  - PA4014
VI  - 48
IP  - suppl 60
4099  - http://erj.ersjournals.com/content/48/suppl_60/PA4014.short
4100  - http://erj.ersjournals.com/content/48/suppl_60/PA4014.full
SO  - Eur Respir J2016 Sep 01; 48
AB  - Introduction: Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death in the world. Currently no causal therapy is available for this devastating disease. In COPD/emphysema the endogenous lung repair capacity is impaired due to decreased canonical WNT/β-catenin signalling in the alveolar epithelium. Canonical and non-canonical (β-catenin independent) WNT signaling pathways are interconnected, meaning that components of one pathway regulates the activity of the other. We investigated if non-canonical WNT signaling could affect β-catenin signaling in alveolar epithelial cells thereby contributing to COPD pathogenesis.Methods: Expression of the non-canonical ligands was analyzed in experimental and human COPD. The potential contribution of WNT-5A to COPD pathogenesis was investigated in vitro using primary human lung fibroblasts (PhF) and alveolar epithelial cells and in vivo in a mouse model of elastase-induced emphysema.Results: Non-canonical WNT-5A is differentially expressed and post-translationally modified COPD. WNT-5A is secreted by PhF, which was enhanced by components of cigarette smoke extract and TGF-β. Treatment of alveolar epithelial cells with WNT-5A impaired canonical WNT/β-catenin signaling. Moreover, mature WNT-5A inhibited WNT/β-catenin driven alveolar epithelial cell proliferation, wound repair and transdifferentiation. Lung-specific overexpression of WNT-5A in vivo aggravated airspace enlargement and loss of functional lung tissue in experimental emphysema.Conclusion: WNT-5A secretion by pulmonary fibroblasts attenuates canonical WNT/β-catenin signaling in the lung epithelium thereby compromising endogenous lung repair in COPD.