TY - JOUR T1 - To determine diagnostic accuracy of gene xpert and sputum Ziehl-Neelsen staining taking sputum culture as gold standard JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2016.PA2779 VL - 48 IS - suppl 60 SP - PA2779 AU - Wasib Shah Y1 - 2016/09/01 UR - http://erj.ersjournals.com/content/48/suppl_60/PA2779.abstract N2 - Background: Pulmonary tuberculosis (PTB) can be diagnosed with various tests including sputum ZN staining, Gene Xpert, and culture. Sputum ZN stain although detects tuberculosis within hours, it is less sensitive. Gene-Xpert is real-time PCR based rapid molecular assay for diagnosing TB. By early detection of tuberculosis in sputum negative patient, GeneXpert can prevent the spread of TB. So we conducted this study in local setup and compared GeneXpert and sputum ZN staining.Objective: To determine the diagnostic accuracy of GeneXpert and sputum ZN staining in predicting tuberculosis taking sputum culture as Gold standard.Methodology: This Cross Sectional Validation Study was conducted at Department of Pulmonology, Military Hospital, Rawalpindi for 6 months. 265 cases were included through Consecutive (non-probability) sampling.Results: Mean age of patients was 45.65±14.91 years. There were 183(69.1%) male and 82(30.9%) female patients. Sensitivity and Specificity of Sputum smear microscopy was 68.48% and 74.07%. While PPV, NPV and Diagnostic accuracy of Sputum Smear microscopy was 85.71%, 50.85% and 70.19% respectively. Sensitivity and Specificity of Gene XPERT was 97.83% and 92.59%. However PPV, NPV and Diagnostic accuracy of Gene XPERT test was 96.77%, 94.94% and 96.23% respectively.Conclusion: Hence it has been concluded through results of this study is that GeneXpert is more accurate and reliable than sputum smear microscopy in predicting pulmonary TB.Key words: Pulmonary TB, Gene Xpert, Sputum culture, sputum smear microscopy, diagnostic accuracy. ER -