RT Journal Article
SR Electronic
T1 Effects of infections on lipidomic profile of bronchial washing (BW) and bronchoalveolar lavage (BAL) after lung transplantation
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP OA3336
DO 10.1183/13993003.congress-2016.OA3336
VO 48
IS suppl 60
A1 Domenica Federica Briganti
A1 Beatrice Aramini
A1 Carla Kim
A1 Robin Chan
A1 Bowen Zhou
A1 Somwmya Sreekanth
A1 Kashif Raza
A1 Hilary Robbins
A1 Lori Shah
A1 Selim Arcasoy
A1 Joshua Sonett
A1 Federica Meloni
A1 Gilbert Di Paolo
A1 Frank D'Ovidio
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/OA3336.abstract
AB Lung transplantation outcomes are challenged by bacterial, viral and fungal infections, noxious triggers that could amplify or promote inflammation and chronic lung allograft dysfunction.We hypothesized the airway lipidome serves as marker of lung infection and related innate immunity. The effects of bacterial, viral and fungal infections on the BW and BAL lipidome from allografts were studied.BW (596) and BAL (199), prospectively collected from 288 pts during surveillance bronchoscopy, were assayed retrospectively by mass-spec for 25 lipid families. Microbiology info was collected. Mann Whitney test used.Bacteria were isolated in 168 BW and 49 BAL, virus in 54 BW and 22 BAL, fungi in 102 BW and 33 BAL. Virus and fungi showed greater BW and BAL total lipid concentration (p<0.05). No difference for bacteria.BW with bacteria had greater (p<0.005): cholesteryl ester (CE), sphingomyelin (SM), N-acylphosphatidylserine; no difference for BAL.BW with virus had greater (p<0.005): monoacylglicerole, ceramide, SM, DihydroSM (dhSM), monosialodihexosylganglioside (GM3), phosphatidylcholine-ether (PCE), phosphatidylethanolamine-p, lysophosphatidylethanolamine-p, lysophosphatidylinositol (LPI), BisMonoacylglyceroPhosphate (BMP); while BAL had greater (p<0.005): dhSM, Lactosylceramide (LacCer).BW with fungi had greater (p<0.005): CE, SM, dhSM, HexosylCeramide, LacCer, GM3, PCE, LPI; while no difference in BAL.The bronchial and broncho-alveolar lipidome of lung allografts is affected by the microbiologic status. Bacteria, virus and fungi have signature lipidomic profile that we speculate associates within the specific bronchial and broncho-alveolar innate immunity.