RT Journal Article
SR Electronic
T1 Genetic homology between bacteria in pus from the pyothorax and bacteria in the oral cavity in patients with pyothorax
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA2630
DO 10.1183/13993003.congress-2016.PA2630
VO 48
IS suppl 60
A1 Rinko Katsuda
A1 Junya Inubushi
A1 Toru Eguchi
A1 Yasuharu Nakahara
A1 Kunihiko Terada
A1 Ryogo Kagami
A1 Yasushi Fukuda
A1 Kiyotaka Onishi
A1 Kenji Hanaoka
A1 Tomohiro Kato
A1 Kouhei Miyake
A1 Yoko Yokoi
A1 Yasuyuki Mizumori
A1 Hiroaki Tsukamoto
A1 Shin Sasaki
A1 Tetsuji Kawamura
A1 Yoshiro Mochizuki
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA2630.abstract
AB Introduction: The bacteria responsible for pyothorax may be derived from the oral cavity; however, it has not been proven yet.Objective: Our aim was to study if there is genetic homology between the bacteria in pus from the pyothorax and the bacteria in the oral cavity using Next-Generation Sequencing technology (NGS) in patients with pyothorax.Methods: Two patients with pyothorax were studied. We aspirated pus from the pyothorax by fine-needle biopsy and sampled the bacteria in the oral cavity with a sponge. Cultured bacteria from the pus were isolated and identified by their 16S rRNA gene sequence. Then, we obtained its whole genome sequence by NGS using the MiSeq (illumina) platform. Based on this sequence and an already-known sequence of the same bacterial species, we generated five specific primers for the isolated pyothorax strain. We amplified total DNA that had been extracted from the oral bacteria using these specific primers by the PCR method and performed DNA sequencing.Results: In both patients, we identified the Streptococcus anginosus group from the pus. In each patient, the sequence of the oral bacterial DNA that had been amplified by specific primers for the pyothorax strain completely matched the DNA sequence of the isolated pyothorax strain, while it did not match already-known sequences of the same bacterial species.Conclusion: This study demonstrated genetic homology between the bacteria in pus from the pyothorax and oral bacteria at the strain level in pyothorax patients. This study proved for the first time that pyothorax is caused by oral bacteria, although the number of cases was limited.