%0 Journal Article %A Veronika Slizen %A Larissa Surkova %T Genotypes of mycobacterium tuberculosis with pre-extended drug resistance in Belarus %D 2016 %R 10.1183/13993003.congress-2016.OA4827 %J European Respiratory Journal %P OA4827 %V 48 %N suppl 60 %X High disease rate of extended and multi-drug resistant tuberculosis (XDR, MDR) in some countries may be associated with the spread of highly virulent genetic variants, such as Beijing, theproportion of which in the TB structure can vary from 30 to 70% depending on the geographical region. The aim was to identify genotypes of pre-XDR strains of MTB, isolated from patients in Belarus.Methods. Genotypes of sixty five pre-XDR strains of MTB were detected by MIRU-VNTR typing method based on the evaluation of differences in 15 VNTR-loci. Mutations in the gene gyrA of MTB were detected by sequencing as well as by GenoType MTBDRsl (HAIN), qPCR with hydrolysis probes and PCR-RFLP analysis with endonucleases BstUI, TaqI, MluI, CseI.Results. Out of 65 pre-XDR isolates, 83% were found to harbour mutations within gyrA. The most common mutations were observed at codon 94 (n =35), where the wild-type aspartic acid was replaced with glycine (Asp94Gly; n =19), alanine (Asp94Ala; n = 7), or tyrosine (Asp94Tyr; n = 7) or histidine (Asp94His; n=1). In one MTB, a double mutation was registered (Asp94Gly and Ala90Val). Eleven FQ-resistant isolates were determined to be wild type at codons 90, 91, 94. It was shown the genotype Beijing was shown to be dominant, comprising 58% of all MTB with pre-XDR. Haarlem and TUR genotypes were detected in 23 and 16% of isolates, respectively.Conclusion. In Belarus, as in other post-Soviet countries, the epidemic processof TB is mainly sustained by genotype Beijing. Continuous circulation of genotype Beijing results in the accumulation of mutations and increases the proportion of MDR and XDR TB. %U