RT Journal Article
SR Electronic
T1 Comparison between mesothelin and fibulin-3 detection in pleural malignant mesothelioma (MPM) patients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA5075
DO 10.1183/13993003.congress-2016.PA5075
VO 48
IS suppl 60
A1 Pinelli, Valentina
A1 Fini, Donatella
A1 Battolla, Enrico
A1 Ferro, Paola
A1 Franceschini, Maria Cristiana
A1 Fontana, Vincenzo
A1 Pistillo, Maria Pia
A1 Roncella, Silvio
A1 Canessa, Pier Aldo
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA5075.abstract
AB Background: MPM is an asbestos-related tumour with a poor prognosis. Pleural effusion (PE) is often the primary manifestation and can be found in about 95% of MPM at diagnosis. PE is obtained by thoracentesis and it may be important for initial diagnosis. A reliable diagnostic biomarker for MPM is an area of intense research. Among the biomarkers, soluble mesothelin-related peptide (SMRP) and fibulin-3 (FBL-3) have received considerable attention. In the present study, we compared the diagnostic performances of SMRP and FBL-3 detection in the same effusions taken at the time of diagnosis.Methods: the study was performed in 33 patients with MPM, 64 with pleural benign lesions (BNG) and 23 with non-MPM pleural metastases (MTS). All patients had developed a PE and underwent thoracentesis. PE were processed by centrifugation and stored at -20°C until SMRP and FBL-3 analysis was performed. All pleural effusion samples were tested in duplicate.Results: patients with MPM were found to have higher SMRP-median levels in effusion than patients with PE form other pathologies (MPM-median = 22.5 nM; Mts+Bng-median = 4.8 nM; P-value < 0.001). In contrast, the FBL-3 levels in MPM were not higher than the levels in non-MPM patients (MPM-median = 63.0 ng/ml; MTS+BNG-median = 72.6 ng/ml; P-value = 0.174).Next, we assessed the diagnostic accuracy of SMRP and FBL-3 biomarkers by using the ROC analysis. The results showed that SMRP outperformed FBL-3.Conclusion: the present study confirms that SMRP is a good diagnostic marker for MPM effusion as opposed to FBL-3 which proved not to be a valuable marker. In addition, FBL-3 does not add any information to the determination of the SMRP in effusion.