TY - JOUR T1 - Cytofluorimetric BAL cells characterization in IPF and in other ILD JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2016.PA3895 VL - 48 IS - suppl 60 SP - PA3895 AU - Ermanno Puxeddu AU - Daniela Fraboni AU - Floriana Bardaro AU - Francesco Buccisano AU - Gabriella Lucà AU - Paola Rogliani Y1 - 2016/09/01 UR - http://erj.ersjournals.com/content/48/suppl_60/PA3895.abstract N2 - Background: BAL studies in IPF have shown increased alveolar macrophage (AM) and polymorphonuclear leukocyte (PMN) numbers and some lines of evidence suggest a role of these inflammatory cells in the pathogenesis of the disease.Aim of the study: to evaluate the pattern of activation of BAL cells by cytofluorimetric analysis in idiopathic pulmonary fibrosis (IPF) in comparison with other interstitial lung diseases (ILD).Methods: 44 consecutive BAL sample performed at the ILD clinic of the University Hospital of Rome Tor Vergata during standard diagnostic workout were evaluated by cytofluorimetry for expression of the following cell surface markers: CD45, CD71, CD206, HLA-DR, CD15, CD14, CD11c. Diagnosis of the 44 cases were eventually reviewed or based according to the 2013 ATS/ERS guidelines.Results: Final diagnosis was IPF in 16 cases, fibrotic NSIP in 6 cases, cellular NSIP in 3 cases, ILD associated to collagen vascular diseases in 6 cases, Hypersensitivity pneumonia in 5 cases, asbestosis in 2 cases, and unclassifiable ILD in 6 cases. Qualitative cytofluorimetric analysis revealed that IPF BAL samples were characterize by a population of CD206, CD11c and HLA-DR very bright cells, that represented the majority of the cellular population in these samples (74±4%). Quantitative analysis revealed that the sum of the recorded mean intensity fluorescence (MIF) for these 3 surface markers (MIF>250 AU) was associated to the diagnosis of IPF with a sensitivity and specificity greater than 90%.Conclusions: Even with the limitation of the small population, this study reinforce the potential role for BAL cytofluorimetry in the diagnostic workout of ILD patients. ER -