RT Journal Article
SR Electronic
T1 Histological changes in smoking and COPD mice with short-term exposure of PM2.5
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3409
DO 10.1183/13993003.congress-2016.PA3409
VO 48
IS suppl 60
A1 Tianyu Zhou
A1 Guangfa Wang
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA3409.abstract
AB Background: Epidemiological studies reported PM2.5 is associated with exacerbation of COPD, but the mechanism is unclear. And evidence about toxicity of PM2.5 on smokers is absence.Objective: To observe the short-term toxicity of PM2.5 to the smoking mice and the COPD mice.Method: Four groups were established: control, LPS as COPD positive control, PM2.5 and LPS+PM2.5. Normal saline, LPS, PM2.5 and a mixture of LPS and PM2.5 were respectively intra-tracheally administered to male C57BL/6J mice once a day on days 0, 7 and 14. The later three groups were exposured to cigarette smoke on days 1-6, 8-13 and 15-28. Sections of left lower lobes were subjected to hematoxylin and eosin (HE) staining. Mean linear intercept(MIL)was used to measure airspace enlargement.Result: Group LPS, PM2.5 and LPS+PM2.5 all showed marked hyperplasia and degeneration of bronchial epithelial cells and alveolar wall destruction with alveolar space enlargement. Secretions and hyperemia were seen in group PM2.5 and PM2.5+LPS, respectively[Figure 1(A)]. MLI had no difference among these three groups, which indicated they had similar degree of emphysema. MLI was significantly higher in the LPS group (p<0.01), PM2.5 group(p<0.05) and LPS+PM2.5 group(p<0.01) than in the control group[Figure 1(B)].Conclude: Short-term exposure of PM2.5 can cause COPD-like changes in smoking mice, and acute inflammatory response in both smoking and COPD mice.