TY - JOUR T1 - Treg depletion reduces bleomycin-induced pulmonary fibrosis by inducing Th17 and other CD4+T cell subset responses in lung JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2016.PA2095 VL - 48 IS - suppl 60 SP - PA2095 AU - Kaustav Chakraborty AU - Arindam Bhattacharyya Y1 - 2016/09/01 UR - http://erj.ersjournals.com/content/48/suppl_60/PA2095.abstract N2 - Idiopathic Pulmonary fibrosis (IPF) is a deadly progressive lung disease with very few treatment options till now. Chronic inflammation and progressive fibrosis of the pulmonary interstitial tissues are the main features for pulmonary fibrosis (PF). Bleomycin (BLM)-induced pulmonary fibrosis (BIPF) is a commonly used mice model in IPF research. We have chosen two measured time-points at days 7 for inflammatory phase and at days 21 for fibrotic phase after BLM instillation. CD4+CD25+FOXP3+ regulatory T cells (Treg) and Th17 cells both are involved in bleomycin-induced lung fibrosis. It has been demonstrated in our study that after Treg depletion, fibrotic condition in pulmonary fibrosis was reduced. The regulatory function of Tregs on Th17 and other CD4+T cell subsets response in bleomycin-induced pulmonary fibrosis remains unexplored. This study aimed to investigate the effects of Treg depletion on Th17, other CD4+T cell subsets, multiple cytokines involved in BIPF. Our data suggests that, Treg depletion reduces BIPF by inducing Th17 and modulating other CD4+T cell subsets such as CD4+CD28+, CD4+CD28-, CD4+CD103+ T cell responses in lung. We found the higher percentage of CD4+T lymphocytes and increased expression of IL-17A and ROR-γT after Treg depletion. So, Treg cells play crucial role in BIPF by attenuating Th17 cell and other CD4+T cell subsets responses in lung. ER -