RT Journal Article
SR Electronic
T1 Differences in the lipidomic profile of bronchial washing (BW) and bronchoalveolar lavage (BAL) in lung transplanted patients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA4633
DO 10.1183/13993003.congress-2016.PA4633
VO 48
IS suppl 60
A1 Briganti, Domenica Federica
A1 Aramini, Beatrice
A1 Kim, Carla
A1 Chan, Robin
A1 Zhou, Bowen
A1 Sreekanth, Somwmya
A1 Raza, Kashif
A1 Robbins, Hilary
A1 Shah, Lori
A1 Arcasoy, Selim
A1 Sonett, Joshua
A1 Meloni, Federica
A1 Di Paolo, Gilbert
A1 D'Ovidio, Frank
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA4633.abstract
AB Introduction Surveillance bronchoscopy with BW and BAL is common practice post lung transplantation, although with limited clinical utility, other than monitoring for infections. The lipid composition of pulmonary surfactant has been studied although no investigation has been performed to differentiate proximal bronchial from distal broncho-alveolar composition.Aim We sought to study the lipidomic profile of BW and BAL of lung allografts.Methods BW (182) and BAL (182) were prospectively collected from 139 patients during the same surveillance bronchoscopies and assayed by mass spec for 25 lipid families. Mann-WHitney test was used.Results BW had greater (p< 0.001) percentage over total lipids of: monoacylglicerole, triacylglicerole, ceramide, sphingomyelin, hexosylceramide, lactosylceramide, phosphatidic acid, phosphatidylcholine, dipalmitoylphosphatidylcholine, phosphatidylcholine ether, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylethanolamine-p, N-acylphosphatidylserine. In contrast BAL had greater (p<0.001) percentage over total lipids of: diacylglycerol, monosialodihexosylganglioside, Bis(Monoacylglycero)Phosphate.Conclusions Our study shows for the first time that the bronch-alveolar lipidome greatly differs from that present in the proximal bronchial tree. These differences could be explained by presence of different types of cells in bronchi rather than in broncho-alveolar district, but also we speculate a differential role of lipids in the innate immunity of the tracheo-bronchial versus the broncho-alveolar district as primary host defence (i.e. antimicrobial; inflammation processes).