PT  - JOURNAL ARTICLE
AU  - Briganti, Domenica Federica
AU  - Aramini, Beatrice
AU  - Kim, Carla
AU  - Chan, Robin
AU  - Zhou, Bowen
AU  - Sreekanth, Somwmya
AU  - Raza, Kashif
AU  - Robbins, Hilary
AU  - Shah, Lori
AU  - Arcasoy, Selim
AU  - Sonett, Joshua
AU  - Meloni, Federica
AU  - Di Paolo, Gilbert
AU  - D'Ovidio, Frank
TI  - Differences in the lipidomic profile of bronchial washing (BW) and bronchoalveolar lavage (BAL) in lung transplanted patients
AID  - 10.1183/13993003.congress-2016.PA4633
DP  - 2016 Sep 01
TA  - European Respiratory Journal
PG  - PA4633
VI  - 48
IP  - suppl 60
4099  - http://erj.ersjournals.com/content/48/suppl_60/PA4633.short
4100  - http://erj.ersjournals.com/content/48/suppl_60/PA4633.full
SO  - Eur Respir J2016 Sep 01; 48
AB  - Introduction Surveillance bronchoscopy with BW and BAL is common practice post lung transplantation, although with limited clinical utility, other than monitoring for infections. The lipid composition of pulmonary surfactant has been studied although no investigation has been performed to differentiate proximal bronchial from distal broncho-alveolar composition.Aim We sought to study the lipidomic profile of BW and BAL of lung allografts.Methods BW (182) and BAL (182) were prospectively collected from 139 patients during the same surveillance bronchoscopies and assayed by mass spec for 25 lipid families. Mann-WHitney test was used.Results BW had greater (p< 0.001) percentage over total lipids of: monoacylglicerole, triacylglicerole, ceramide, sphingomyelin, hexosylceramide, lactosylceramide, phosphatidic acid, phosphatidylcholine, dipalmitoylphosphatidylcholine, phosphatidylcholine ether, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylethanolamine-p, N-acylphosphatidylserine. In contrast BAL had greater (p<0.001) percentage over total lipids of: diacylglycerol, monosialodihexosylganglioside, Bis(Monoacylglycero)Phosphate.Conclusions Our study shows for the first time that the bronch-alveolar lipidome greatly differs from that present in the proximal bronchial tree. These differences could be explained by presence of different types of cells in bronchi rather than in broncho-alveolar district, but also we speculate a differential role of lipids in the innate immunity of the tracheo-bronchial versus the broncho-alveolar district as primary host defence (i.e. antimicrobial; inflammation processes).