TY - JOUR T1 - Exercise-induced bronchoconstriction: New cellular and molecular mechanisms evaluated in 80 male marathon runners JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2016.PA5032 VL - 48 IS - suppl 60 SP - PA5032 AU - Rodolfo de Paula Vieira AU - Ana Paula Sierra AU - Manoel C. Oliveira-Junior AU - Francine M. Almeida AU - Marino Benetti AU - Rodrigo Oliveira AU - Soraia Nogueira AU - Isabella Santos Genaro AU - Beatriz Mangueira Saraiva-Romanholo AU - Milton Arruda Martins AU - Nabil Ghorayeb AU - Maria Fernanda Cury-Boaventura AU - João Bosco Pesqueiro AU - Maria Augusta Peduti Dal Molin Kiss Y1 - 2016/09/01 UR - http://erj.ersjournals.com/content/48/suppl_60/PA5032.abstract N2 - Exercise is a common triggers of bronchoconstriction in asthmatic and non-asthmatics, mainly after strenuous exercise. The cellular and molecular mechanisms involved in this response is poorly understood. Thus, this study investigated whether the bronchoconstriction observed immediately after completing a marathon by 31 male marathon runners occurred due to increased levels of exhaled nitric oxide (eNO), epithelial injury (increased epithelial detachment) and modulation of pulmonary immune system. The results were presented as mean±SD (pre versus post-marathon) of paired t test analysis. Immediately after marathon, FEV1 was significantly reduced (p<0.009), which was followed by increased levels of eNO (20,21±11,14 vs 34,56±19,06 ppb; p<0.0007), increased numbers of total cells x 104/mL (143,45±15,52 vs 266,14±43,35; p<0.001), neutrophils (13,33±9,89 vs 60,5±13,16; p<0.01); macrophages (21,23±5,91 vs 54±13,6; p<0.01); epithelial cells (113,01±11,73 vs 200,74±31,55; p<0.01) in induced sputum. In addition, marathon running has impaired the innate and acquired pulmonary immune system, as demonstrated by reduced levels (pg/mL) of IL-12p40 (p<0.01), IL-17 (p<0.01) and IL-23 (p<0.03), IL-1ra (p<0.02) and TSLP (p<0.0048). Reduced TSLP levels indicates epithelial deactivation, which correlates with increased number of epithelial cells in induced sputum, suggesting epithelial death and detachment. Therefore, this study shows for the first time that in experienced marathon runners, reduced FEV1 post-marathon is followed by increased levels of exhaled nitric oxide, airway inflammation, epithelial injury and abrogation of the pulmonary immune system. ER -