RT Journal Article
SR Electronic
T1 Cytokine profile in bronchoalveolar lavage fluid of fibrotic idiopathic intersticial pneumonias compared to fibrotic intersticial pneumonias in connective tissue diseases
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA4884
DO 10.1183/13993003.congress-2016.PA4884
VO 48
IS suppl 60
A1 Martina Kolbeková
A1 Martina Šterclová
A1 Magdalena Smetáková
A1 Peter Paluch
A1 Jan Anton
A1 Jelena Skibová
A1 Martina Vašáková
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA4884.abstract
AB Objectives: Fibroproliferative type of healing of lung tissue can be found in idiopathic intersticial pneumonias (fIIPs),but also in interstitial pneumonias accompanying connective tissue diseases (fIP-CTDs).Aim: To prove, if there is a difference between pathogenetic pathways of fIIPs and fIP-CTDs by comparing proteinaceous parameters in bronchoalveolar lavage fluid (BALF).Material and methods: We compared 2 groups:22 patients with fIIP (5women/17 men, mean age 68.3±5.4,17smokers/5non-smokers), 22 patients with fIP-CTDs (14 women/8 men, mean age 61.82±13.0,10smokers/12non-smokers). In fIIPs 4 patients had NSIP and 18 IPF. In fIP-CTDs 12 patients had NSIP,9 UIP and 1 AIP pattern. All patients underwent bronchoalveolar lavage at the time of diagnosis. BALF concentrations of matrix metalloproteinase-7(MMP7), interleukin-4 receptor(IL4-R), proteinase activated receptor 2(PAR-2), B-cell activating factor (BAFF) and tumor necrosis factor-α(TNFα) were measured by ELISA (Cloud nad Clone Corp.,USA). We used two group t-test and Chi-square test in frequency tables to state statistically significant data.Results: Patiens with fIPs-CTDs were significantly younger (p<0.05), women (p<0.01) and also smokers prevailed (p<0.05). We found significantly higher concentrations of TNF-α (3.04±2.19 vs. 1,49±0,78 pg/ml, p<0.01), lower concentrations of MMP7 (2.07±1.45 vs. 3,26±2,09 pg/ml, p<0.05) in BALF in fIP-CTDs.Conclusion: Higher levels of TNF-α in BALF in fIP-CTDs might reflect inflammatory background of CTDs. Higher levels of MMP-7 in BALF in fIIPs can represent accented fibroproliferative healing in fIIPs compared to fIP-CTDs.