PT  - JOURNAL ARTICLE
AU  - Martina Kolbeková
AU  - Martina Šterclová
AU  - Magdalena Smetáková
AU  - Peter Paluch
AU  - Jan Anton
AU  - Jelena Skibová
AU  - Martina Vašáková
TI  - Cytokine profile in bronchoalveolar lavage fluid of fibrotic idiopathic intersticial pneumonias compared to fibrotic intersticial pneumonias in connective tissue diseases
AID  - 10.1183/13993003.congress-2016.PA4884
DP  - 2016 Sep 01
TA  - European Respiratory Journal
PG  - PA4884
VI  - 48
IP  - suppl 60
4099  - http://erj.ersjournals.com/content/48/suppl_60/PA4884.short
4100  - http://erj.ersjournals.com/content/48/suppl_60/PA4884.full
SO  - Eur Respir J2016 Sep 01; 48
AB  - Objectives: Fibroproliferative type of healing of lung tissue can be found in idiopathic intersticial pneumonias (fIIPs),but also in interstitial pneumonias accompanying connective tissue diseases (fIP-CTDs).Aim: To prove, if there is a difference between pathogenetic pathways of fIIPs and fIP-CTDs by comparing proteinaceous parameters in bronchoalveolar lavage fluid (BALF).Material and methods: We compared 2 groups:22 patients with fIIP (5women/17 men, mean age 68.3±5.4,17smokers/5non-smokers), 22 patients with fIP-CTDs (14 women/8 men, mean age 61.82±13.0,10smokers/12non-smokers). In fIIPs 4 patients had NSIP and 18 IPF. In fIP-CTDs 12 patients had NSIP,9 UIP and 1 AIP pattern. All patients underwent bronchoalveolar lavage at the time of diagnosis. BALF concentrations of matrix metalloproteinase-7(MMP7), interleukin-4 receptor(IL4-R), proteinase activated receptor 2(PAR-2), B-cell activating factor (BAFF) and tumor necrosis factor-α(TNFα) were measured by ELISA (Cloud nad Clone Corp.,USA). We used two group t-test and Chi-square test in frequency tables to state statistically significant data.Results: Patiens with fIPs-CTDs were significantly younger (p<0.05), women (p<0.01) and also smokers prevailed (p<0.05). We found significantly higher concentrations of TNF-α (3.04±2.19 vs. 1,49±0,78 pg/ml, p<0.01), lower concentrations of MMP7 (2.07±1.45 vs. 3,26±2,09 pg/ml, p<0.05) in BALF in fIP-CTDs.Conclusion: Higher levels of TNF-α in BALF in fIP-CTDs might reflect inflammatory background of CTDs. Higher levels of MMP-7 in BALF in fIIPs can represent accented fibroproliferative healing in fIIPs compared to fIP-CTDs.