PT - JOURNAL ARTICLE AU - Tatiana Tumanova AU - Viacheslav Aleksandrov TI - Lipolysaccharide alters the pattern of breathing and Hering-Breuer reflexes in anaesthetized rats AID - 10.1183/13993003.congress-2016.PA2293 DP - 2016 Sep 01 TA - European Respiratory Journal PG - PA2293 VI - 48 IP - suppl 60 4099 - http://erj.ersjournals.com/content/48/suppl_60/PA2293.short 4100 - http://erj.ersjournals.com/content/48/suppl_60/PA2293.full SO - Eur Respir J2016 Sep 01; 48 AB - Systemic inflammatory response caused by infectious agents leads to respiratory dysfunctions apparently due to hypercytokinemia. Lipolysaccharide (LPS) is component of bacterial wall which causes a strong induction of cytokines. The purpose of present study is to test the hypothesis that the LPS-induced hypercytokinemia leads to changes as in breathing pattern and respiratory reflexes. The experiments were performed on the two sets of the urethane anaesthetized Wistar male rats. Pneumotachograph was used to calculate the tidal volume (VT), respiratory frequency (f) and minute ventilation (VE). The duration of inspiration and expiration were determined by the electromyogram of diaphragm. Methods of functional vagotomy and vagal apnea had been used to measure the strength of Hering-Breuer reflexes. Control experiments (n=10) had demonstrated that pattern of breathing and Hering-Breuer reflexes were stable for four hours of recording. Intravenous administration (n=12) of 1 ml of saline containing LPS (7 mg/kg) led to a significant (P<0,05) increase in VT and VE while the f increased insignificantly. In addition there was an increase in the electrical activity of the diaphragm. On the other hand there was a gradual weakening of Hering-Breuer reflexes which are involved in the regulation of breathing depth and rate. The results obtained confirm the hypothesis which suggests that the hypercytokinemia effects on breathing pattern and respiratory reflexes. This may be the cause of respiratory dysfunction accompanying systemic inflammatory response.The study was supported by Russian Science Foundation (RScF) grant No. 15-15-00119.