RT Journal Article
SR Electronic
T1 First evaluation of QuantiFERON-TB Gold Plus performance in contact screening
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1411
OP 1419
DO 10.1183/13993003.00510-2016
VO 48
IS 5
A1 Lucia Barcellini
A1 Emanuele Borroni
A1 James Brown
A1 Enrico Brunetti
A1 Daniela Campisi
A1 Paola F. Castellotti
A1 Luigi R. Codecasa
A1 Federica Cugnata
A1 Clelia Di Serio
A1 Maurizio Ferrarese
A1 Delia Goletti
A1 Marc Lipman
A1 Paola M.V. Rancoita
A1 Giulia Russo
A1 Marina Tadolini
A1 Elisa Vanino
A1 Daniela M. Cirillo
YR 2016
UL http://erj.ersjournals.com/content/48/5/1411.abstract
AB Identifying latently infected individuals is crucial for the elimination of tuberculosis (TB). We evaluated for the first time the performance of a new type of interferon-γ release assay, QuantiFERON-TB Plus (QFT-Plus), which includes an additional antigen tube (TB2), stimulating both CD4+ and CD8+ T-cells in contacts of TB patients.Contacts were screened for latent TB infection by tuberculin skin test, QFT-Plus and QuantiFERON-TB Gold in Tube (QFT-GIT).In 119 TB contacts, the overall agreement between QFT-Plus and QFT-GIT was high, with a Cohen's κ of 0.8. Discordant results were found in 12 subjects with negative QFT-GIT and positive QFT-Plus results. In analyses of markers of TB exposure and test results, the average time spent with the index case was the strongest risk factor for positivity in each of these tests. The difference in interferon-γ production between the two antigen tubes (TB2−TB1) was used as an estimate of CD8+ stimulation provided by the TB2. TB2−TB1 values >0.6 IU·mL−1 were significantly associated with proximity to the index case and European origin.QFT-Plus has a stronger association with surrogate measures of TB exposure than QFT-GIT in adults screened for latent TB infection. Interferon-γ response in the new antigen tube used an indirect estimate of specific CD8+ response correlates with increased Mycobacterium tuberculosis exposure, suggesting a possible role in identifying individuals with recent infection.QuantiFERON-TB Plus improved the diagnostic accuracy for latent TB infection in the setting of contact screening http://ow.ly/2Az0300SDg3