RT Journal Article
SR Electronic
T1 Airway dysbiosis: <em>Haemophilus influenzae</em> and <em>Tropheryma</em> in poorly controlled asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 792
OP 800
DO 10.1183/13993003.00405-2015
VO 47
IS 3
A1 Jodie L. Simpson
A1 Joshua Daly
A1 Katherine J. Baines
A1 Ian A. Yang
A1 John W. Upham
A1 Paul N. Reynolds
A1 Sandra Hodge
A1 Alan L. James
A1 Philip Hugenholtz
A1 Dana Willner
A1 Peter G. Gibson
YR 2016
UL http://erj.ersjournals.com/content/47/3/792.abstract
AB Asthma is a chronic inflammatory disorder of the airways where bacteria may act as protagonists of chronic inflammation. Little is known about the relation of airway inflammation to the presence of specific bacterial taxa. We sought to describe the sputum microbiome in adults with poorly controlled asthma.DNA was extracted from induced sputum and microbial communities were profiled using 16S rRNA pyrosequencing. Bacterial species were characterised, and the relationship between microbial populations, asthma inflammatory subtypes and other covariates was explored. Real-time PCR was used to identify Tropheryma whipplei and Haemophilus influenzae in sputum.Adults with neutrophilic asthma had reduced bacterial diversity and species richness. Tropheryma was identified and confirmed with real-time PCR in 12 (40%) participants. Haemophilus occurred most often in a group of younger atopic males with an increased proportion of neutrophils. PCR confirmed the presence of H. influenzae in 35 (76%) participants with poorly controlled asthma.There are phenotype-specific alterations to the airway microbiome in asthma. Reduced bacterial diversity combined with a high prevalence of H. influenzae was observed in neutrophilic asthma, whereas eosinophilic asthma had abundant T. whipplei.There are phenotype-specific alterations to the airway microbiome in asthma which may modulate local inflammation http://ow.ly/UbB9k