TY - JOUR T1 - Asymmetric dimethylarginine (ADMA) in exhaled breath condensate (EBC) of children with cystic fibrosis (CF) JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA1305 VL - 46 IS - suppl 59 SP - PA1305 AU - Francesca Lucca AU - Mirco Ros AU - Silvia Carraro AU - Giuseppe Giordano AU - Liviana Da Dalt AU - Eugenio Baraldi Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA1305.abstract N2 - Background: ADMA competitively inhibits nitric oxide synthase (NOS) and its levels in specimens from murine models and asthmatic patients (pts) are related to inflammation and oxidative stress.In CF increased arginase, reduced NO production and NOS expression and higher ADMA in sputum than in healthy controls (HC) are reported.Aim: To determine ADMA and related aminoacids levels in EBC in pediatric CF pts and to compare stable and exacerbated pts to each other and to HC.Methods: 34 CF pts (21 stable, 13 exacerbated) were included (5-18 yrs, both sexes, mean FEV1 in last 6 month >70%pred); systemic steroids in the last month, B. Cepacia colonization, lung transplantation, allergic aspergillosis were exclusion criteria. 16 HC (5-18 yrs, non-smokers, without chronic or recent acute respiratory diseases) were enrolled.Visit, spirometry and EBC collection were performed at enrollment (T1) and at the end of the antibiotic treatment (T2) in exacerbated pts.EBC analysis was performed with Ultrahigh-Performance Liquid Chromatography coupled with tandem mass spectrometry after on line sample trapping and enrichment through automated auto sampler.Results: ADMA, NMMA, arginine and homoarginine absolute levels and homoarginine ratio to tyrosine were higher in the EBC of HC. No difference was found between stable and exacerbated pts at T1 nor between exacerbated pts at T1 and T2. No correlation between metabolites and spirometry changes from T1 to T2 was found.Conclusion: ADMA and related aminoacids were simultaneously measured in EBC from CF pts for the first time. At present no clinical use of ADMA in EBC from CF pts can be suggested, but further studies are needed to enlighten its role. ER -