TY - JOUR T1 - Effects of bone-marrow derived mononuclear cells from silicotic and healthy donors in experimental silicosis JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA942 VL - 46 IS - suppl 59 SP - PA942 AU - Helena Oliveira AU - Elga Bandeira AU - Miquéias Lopes-Pacheco AU - Johnatas Silva AU - Christina Takyia AU - Patrícia Rocco AU - Marcelo Morales Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA942.abstract N2 - Background: Silicosis is a chronic lung disease and with no effective therapy.Aim: To investigate the effects of bone-marrow mononuclear cells (BMMCs) obtained from healthy and silicotic donors on lung morpho-function in experimental silicosis.Methods: C57BL/6 mice were randomly divided in control (C) and silicosis (SIL) groups, intratracheally instilled with saline (50mL) or silica (20mg/50mL), respectively, and then treated intravenously with saline (Sal) or BMMCs (2x106) from healthy (ht) and silicotic (sc) mice.Results: SIL-Sal group showed higher lung static elastance (Est) (48.7±1.1cmH2O.ml-1), resistive (DP1) (1.4±0.1cmH2O) and viscoelastic (DP2) (1.2±0.1cmH2O) pressures than C group (33.7±1.4cmH2O.ml-1; 0.8±0.1cmH2O; 0.8±0.1cmH2O, respectively). Est, DP1, and DP2 were lower in SIL-BMMCs-ht (40.2±0.5cmH2O.ml-1; 1.1±0.1cmH2O; 0.8±0.1cmH2O) and SIL-BMMCs-sc (41.8±0.9 cmH2O.ml-1; 1.2±0.1cmH2O; 1.0±0.1cmH2O) than SIL-Sal group. The degree of apoptosis was higher in SIL-Sal (8.4±1.1%) than SIL-BMMCs-ht (2.5±0.4%) and SIL-BMMCs-sc (3.0±0.2%). SIL-Sal group presented a higher number of mononuclear (MN) and polymorphonuclear (PMN) cells in septae (42.6±1.0%, 8.2±0.7%) and nodules (38.8±0.5%; 11.3±0.7%) than SIL-BMMCs-ht (MN:38.3±1.3% and 36.3±0.8%, PMN: 5.1±0.8%, 7.2±0.9%, respectively) and SIL-BMMCs-sc (MN: 35.3±0.6% and 34.2±0.8%, PMN: 5.8±1.0% and 6.8±0.8%, respectively). SIL-Sal group showed higher fraction area of nodules (58.8±4.3%) than SIL-BMMCs-ht (32.8±2.7%) and SIL-BMMCs-sc (29.0±3.7%).Conclusion: Therapy with BMMCs-sc and BMMCs-ht led to similar improvement in lung mechanics and histology in the current experimental silicosis.Fundings: FAPERJ, CNPq, MS-DECIT. ER -