PT  - JOURNAL ARTICLE
AU  - Edith Silbernagel
AU  - Wolfgang Gesierich
AU  - Michael Lindner
AU  - Alicia Morresi-Hauff
AU  - Juergen Behr
AU  - Frank Reichenberger
TI  - Imaging pattern in interstitial lung diseases using probe based confocal laser endomicroscopy (pCLE)
AID  - 10.1183/13993003.congress-2015.PA4859
DP  - 2015 Sep 01
TA  - European Respiratory Journal
PG  - PA4859
VI  - 46
IP  - suppl 59
4099  - http://erj.ersjournals.com/content/46/suppl_59/PA4859.short
4100  - http://erj.ersjournals.com/content/46/suppl_59/PA4859.full
SO  - Eur Respir J2015 Sep 01; 46
AB  - Confocal laser endomicroscopy allows in vivo visualisation of the alveoli during bronchoscopy. We used pCLE (Cellvizio®, Mauna Kea Technology Inc.) to characterise imaging pattern in patients with histology proven interstitial lung disease.We included 21 therapy naive patients (16 male, age 72 +/- 6 years), who underwent bronchoscopy for workup of interstitial lung disease. PCLE images were obtained in 2 affected lung segments according to HR-CT scan, followed by cryobiopsies in the identical area. Diagnoses were made in a multidisciplinary panel. PCLE patterns were semi-quantitatively described according to degree of distortion of the alveolar pattern, density of alveolar structures and presence of consolidations and loaded alveolar macrophages. The assessment was performed by 2 investigators blinded to histology. In amiodarone lung disease loaded AM were predominantly present, whereas IPF showed severe distortion and increased density. NSIP was characterised by increased density but preserved alveolar structure, while COP presented with moderate distortion and densitiy of the alveolar pattern. Consolidations were present in COP and chronic HP. There was a weak correlation between degree of loaded AM and BAL eosinophils (r=0,40), between degree of distortion and BAL neutrophils (r=0,43), and a weak negative correlation between distortion and VC %pred (r=-0,41) and pCO2 and degree of loaded AM (r= -0,47). PCLE might serve as an adjunctive bronchoscopic imaging method in the differential diagnosis and assessment of ILD.Diagnosisndistortiondensityconsolidationloaded AMCOP4xxxxxxxNSIP7xxx(x)0IPF5xx(x)xxx00Amiodarone lung3xx0xxxchronic HP2xxxxx0