RT Journal Article
SR Electronic
T1 Differentiate late-onset asthma and COPD by CD4+ T memory cells in peripheral blood
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3612
DO 10.1183/13993003.congress-2015.PA3612
VO 46
IS suppl 59
A1 Zhihong Chen
A1 Kan Xu
A1 Xinjun Tang
A1 Zhihui Min
A1 Xiangdog Wang
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/PA3612.abstract
AB Objectives: Memory T cells has the ability of initiating more effective immune response against antigen. We studied the phenotype and cell signaling pathway in CD4+ memory T cells in late-onset asthma and COPD and tried to find a new way help differentiating these two diseases.Methods: 42 subjects were enrolled, which included 15 late-onset asthma, 15 COPD and 12 healthy control. CD4+ T cells were purified and cultured under neutralizing, Th2, Th2 plus LPS, Th2 plus HDM conditions. On day 3, CD45RO expression was detected. On day 6, the level of intracellular cytokines was measured. The freshly isolated PBMC on day 0 were stimulated by IL27 or IL4 to test STAT1 and STAT6 phosphorylation by flow.Results: late-onset Asthma and COPD have more CD4+T memory cells. CD4+ memory T cells of Asthma released more IL5 and IL17 (3.5% vs 0.6%; 2.2% vs 1.2%) respectively , less IFN-r(15.3% VS 22.5%) compared to healthy control. The cytokine profile of COPD was more like healthy control. Small amount of IL10 was found ,with no differences among 3 groups (0.9%, 0.5%, 0.7%). IL27-induced STAT1 phosphorylation and IL4-induced STAT6 phosphorylation were measured.The ratios of ΔSTAT1-Py/ΔSTAT6-Py were 0.55, 1.09, 1.63 in late-onset asthma , COPD and healthy respectivelyConclusions: There are more CD4+ memory T cells in the peripheral circulation system in asthma and COPD. The cytokine production profile of asthma was different from COPD, more Th2-type cytokine released. The balancing between STAT1-Py/STAT6-Py was prone to STAT6-Py in asthma. These differences might be used to develop a new way to distinguish the hardly differentiated disease, especially late-onset asthma and asthma- COPD overlap syndrome.