PT - JOURNAL ARTICLE AU - Bao-ping Tian AU - Li-xia Xia AU - Fen Lan AU - Ping-li Wang AU - Zheng-qiang Bao AU - Zhi-Hua Chen AU - Song-min Ying AU - Hua-Hao Shen TI - Bcl-2 antagonist ABT-737 attenuates corticosteroid-insensitive neutrophilic airway inflammation AID - 10.1183/13993003.congress-2015.PA3906 DP - 2015 Sep 01 TA - European Respiratory Journal PG - PA3906 VI - 46 IP - suppl 59 4099 - http://erj.ersjournals.com/content/46/suppl_59/PA3906.short 4100 - http://erj.ersjournals.com/content/46/suppl_59/PA3906.full SO - Eur Respir J2015 Sep 01; 46 AB - Background: Resolution of inflammatory cells in inflamed tissues contributes to alleviate disease. Number of neutrophil is found increased in patients with acute or persistent asthma, especially in poor response to inhaled corticosteroids.Objectives: We thought to explore a new strategy in the treatment of corticosteroid-insensitive neutrophilic asthma by the induction of inflammatory cells apoptosis.Methods and results: Here, using cultured bronchoalveolar lavage (BAL) cells and murine models, we demonstrated that eosinophils and neutrophils in BAL fluid form allergen-induced airway inflammation showed the higher viability and prolonged lifetime. We observed the elevated expression of pro-survival protein Bcl-2 on these inflammatory cells. We also provided the aggravated inflammation response and prolonged life of inflammatory cells in Bcl-2 overexpressed asthmatic mice. Activated neutrophil and neutrophil-dominated airway inflammation indicated resistance in response to corticosteroid treatment. By contrast, eosinophilic and neutrophilic airway inflammation treated with ABT-737/199 suggested the enhanced apoptosis level of inflammatory cells and thus exerted the inhibitory effect in inflammation.Conclusions: These findings implicateABT-737/199 is a promising medicine used in the treatment of airway inflammation especially the corticosteroids-insensitive neutrophilic airway inflammation, this possibly due to the enhanced death of airway inflammatory cells by combination with the higher pro-survival protein Bcl-2.