RT Journal Article
SR Electronic
T1 Hydrogen sulphide attenuates lung inflammation caused by resistive breathing
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA2162
DO 10.1183/13993003.congress-2015.PA2162
VO 46
IS suppl 59
A1 Athanasia Pavlidou
A1 Constantinos Glynos
A1 Fotis Perlikos
A1 Dimitris Toumpanakis
A1 Zongmin Zhou
A1 Christina Magkou
A1 Andreas Papapetropoulos
A1 Theodoros Vassilakopoulos
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/PA2162.abstract
AB Background & Objectives: During exacerbations of chronic obstructive pulmonary disease (COPD), increased airway inflammation and the accompanying bronchoconstriction lead to significant airway narrowing, i.e. resistive breathing (RB).To isolate the effects of mechanical stressor (i.e. RB) from the accompanying lung inflammation we developed an experimental model of RBthat exhibits both increased inspiratory and expiratory airway resistance, via tracheal banding. Since hydrogen sulphide (H2S) plays an important role in vasodilation and inflammation we hypothesized that the administration of a H2S fast releasing donor, Na2S, will attenuate the lung inflammation.Methods: C57BL/6 mice were studied: 1. Sham operated (controls) 2. Tracheal banded (TB) mice (tracheal surface area restricted to 50% of initial) 3. Sham operated mice treated with injected Na2S (10mg/kg) 4. Mice treated with injected Na2S 20 min prior to tracheal banding.Results: The proteinexpression of the three H2S synthesizing enzymes, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulphurtransferase (3MST), was the same between the TB and sham operated group in the lung. However, the lung mRNA level of CBS was decreased by 57% in TB mice (p=0.009). Finally interleukin IL-6 and IL-1β, were attenuated by 50% (p=0.004)and 45% (p=0.02)in the group of TB mice treated with Na2S compared to the TB mice, whereas the expression of TNF-α was the same among the groups.Conclusions: H2S ameliorates lung inflammation caused by airway narrowing. Our model of TB might offer new insights by which resistive breathing can enhance inflammation in obstructive lung disease.