TY - JOUR T1 - Genomic comparison of fibrotic rheumatoid arthritis-associated ILD and idiopathic pulmonary fibrosis JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA3794 VL - 46 IS - suppl 59 SP - PA3794 AU - Lei Wang AU - Paula Walters AU - Asha Nair AU - Jin Jen AU - Andrew Limper AU - Eric Matteson AU - Diane Grill AU - Robert Vassallo Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA3794.abstract N2 - While rheumatoid arthritis (RA) associated ILD (UIP pattern) is clinically very similar to idiopathic UIP (idiopathic pulmonary fibrosis or IPF), limited information exists regarding genomic signatures of RA-ILD compared with IPF. To study this, we obtained frozen lung biopsy specimens from the National Institutes of Health Lung Tissue Research Consortium project, and extracted RNA from 20 control lung specimens, 20 IPF samples, and 5 RA-lung biopsies from patients with diffuse lung involvement. Samples were submitted for mRNA sequencing using the Illumina TruSeq mRNA approach for Next Generation Sequencing analysis. Samples were processed using the Mayo analysis pipeline, MAP-RSeq, and differentially expressed genes were obtained using edgeR. Expression for tumor necrosis factor (TNF), granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-1 (IL-1), and interferon-gamma (IFNγ), RANTES, CCR5, CD40L, and interleukin-6 receptor (IL-6R) were upregulated in RA vs IPF tissues. Conversely, mRNA expression for matrix genes (collagen-1 and -3) and matrix metalloproteinases (MMP1, 2, 9, 13) were upregulated in IPF compared to RA lung specimens. These preliminary findings outline key differences in immune and tissue remodeling genes between RA-associated and idiopathic UIP, and provide insight into molecular mechanisms of fibrotic lung disease development in RA. Supported by a Rheumatology Research Foundation grant to RV. ER -