RT Journal Article
SR Electronic
T1 Transcriptome analysis in endobronchial epithelial lining fluid compared to bronchoalveolar lavage in idiopathic pulmonary fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3053
DO 10.1183/13993003.congress-2015.PA3053
VO 46
IS suppl 59
A1 Nicolas Kahn
A1 Martin Granzow
A1 Michael Meister
A1 Ralf Eberhardt
A1 Thomas Muley
A1 Eva Baroke
A1 Ute Oltmanns
A1 Felix J.F. Herth
A1 Michael Kreuter
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/PA3053.abstract
AB Bronchoscopic Microsampling (BMS) of endobronchial epithelial lining fluid (EELF) might be a potent method for assessing novel molecular biomarkers to improve diagnostics and risk assessment in interstitial lung diseases. In contrast to BAL, EELF can be collected highly localization-specific in the airway of areas with active disease.Aim of this study was to analyze the differences between the transcriptome expression patterns in EELF and BAL samples from patients with IPF.EELF was collected from a total of 14 patients by BMS from subsegmental bronchi. BAL from the same patients was sampled from the middle lobe or lingula. Total RNA extraction was performed using AllPrep DNA/RNA Mini Kit (Qiagen). The quantity of total RNA was measured by spectrophotometer (NanoDrop Technologies). The quality of total RNAs was assessed with Agilent 2100 Bioanalyzer. Transcriptome expression analysis was performed with GeneChip® miRNA 3.0 and with Human Transcriptome Array (HTA) 2.0 (both Affymetrix).All patients underwent BMS and BAL sampling without complications. TotalRNA extraction was reliably performed with all EELF and BAL samples. A total of 40 samples were analyzed for microRNA and mRNA expression. Matched BAL and EELF samples of IPF patients showed 51 highly differentially expressed microRNAs. mRNA expression analysis of BAL and EELF samples also revealed a clear differential expression between both sampling materials in IPF patients.A significant differential transcriptome expression was detected between BAL and EELF samples of patients with IPF. This indicates that EELF could be an additional biomaterial worth screening for potential biomarkers in IPF.