TY - JOUR T1 - Effects of LTRA on T cell-induced bronchoconstriction in the mice JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA3568 VL - 46 IS - suppl 59 SP - PA3568 AU - Akio Mori AU - Satoshi Kouyama AU - Miyako Yamaguchi AU - Y.O. Iijima AU - Akemi Ohtomo-Abe AU - Takayuki Ohtomo AU - Natsumi Saito AU - Arisa Kinoshita AU - Kentaro Watarai AU - Chihiro Mitsui AU - Chiyako Oshikata AU - Kiyoshi Sekiya AU - Takahiro Tsuburai AU - Mamoru Ohtomo AU - Yuma Fukutomi AU - Masami Taniguchi AU - Osamu Kaminuma Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA3568.abstract N2 - Aims: To investigate a role of helper T (Th) cells in asthma, T cell-transfer model was analyzed for late phase asthmatic response.Methods: Ovalbumin (OVA) specific Th clones were derived from either the regional lymphnodes of Balb/c mice immunized with OVA/CFA or splenocytes of DO11.10 transgenic mice expressing T cell receptor specific for OVA/H-2d. Th clones were adoptively transferred into unprimed mice. After antigen challenge, airway resistance was continuously monitored by either unrestrained whole body plethysmography(BUXCO) or resistance/compliance analyzer under anesthetized condition. Bronchoalveolar lavage analysis was performed 48 hr after OVA challenge. Supernatants of stimulated Th clones were analyzed for contractile activity using collagen gels embedded with murine primary bronchial smooth muscle cells. Effects of H1R and LTR1 antagonist were analyzed both in vitro and in vivo.Results: When unprimed mice were transferred with Th clones, T5-1, T6-2, T6-4, and T6-7, Penh values were significantly increased 6 hr after challenge with OVA or T cell epitope peptide, OVA323-339. In contrast, mice transferred with other Th clones, BF7, T6-1, or T6-10 did not show any change. Airflow limitation was confirmed by a direct measurement of airway resistance under anesthetized, restrained, and intubated conditions. Contractile activity was detected in the supernatants of T6-2 stimulated with immobilized anti-CD3. T cell-induced contraction was not affected by H1R or LTR1 antagonist.Conclusion: Activation of Th cells resulted in an airflow limitation besides eosinophilic inflammation, AHR, and mucous hyperplasia. T cell-derived bronchoconstrictor might be a target for treatment-resistant asthma. ER -