RT Journal Article
SR Electronic
T1 Adenylate cyclase-associated protein 1 is associated with metastasis of non-small cell lung cancer especially in brain metastasis patients and promotes the lung cancer cell proliferation and migration in vitro as well as its growth and metastasis in vivo by the signaling pathways of limk1-cofilin
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP OA4978
DO 10.1183/13993003.congress-2015.OA4978
VO 46
IS suppl 59
A1 Changhui Wang
A1 Shuanshuan Xie
A1 Min Tan
A1 Xiaolian Song
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/OA4978.abstract
AB Objective: This study was designed to predict non-small cell lung cancer metastasis and explore mechanism of lung cancer cell proliferation, metastasis and migration in vitro and vivo.Methods: Firstly, Adenylate cyclase-associated protein 1, CAP1 were determined by real-time PCR and western blot analysis and IHC in 82 lung cancer specimens. Secondly, BM group (n =50) and non-BM group (n =70) were treated and followed up by 4 years. Thirdly, we characterized the expression of CAP1 in lung cell by using Western blot and RT-PCR analyses. In addition, Si-CAP1, empty vector, over-CAP1 in cultured A549,95-C,95-D by using MTT, wound Healing, Transwell invasive, Western blot, and IHC analyses and nude mouse.Results: We provided evidence that overexpression of CAP1 in lung cancer cells, particularly at BM [figure 1]. In addition,These findings suggest that CAP1 promotes the lung cancer cell proliferation and migration in vitro as well as its growth and metastasis in vivo via LIMK/cofilin I [figure 2]. Conclusion: CAP1 is associated with metastasis of NSCLC especially in BM patients and promotes the lung cancer cell proliferation and migration in vitro and vivo by limk1-cofilin.