TY - JOUR T1 - Transforming growth factor B1 polymorphism and serum levels in interstitial lung diseases JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA4854 VL - 46 IS - suppl 59 SP - PA4854 AU - Rana El-Helbawy AU - Sami Eldahdoh AU - Rania Elshzly AU - Ashraf Dawood Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA4854.abstract N2 - Background: Interstitial lung diseases (ILD) are a heterogenous group of diffuse parenchymal disorders characterized by different grades of fibrosis and inflammation. Studies have demonstrated associations between cytokine gene polymorphisms and the risk of ILD. TGF-β1is one of the key cytokines involved in the pathogenesis of pulmonary fibrosis.Aim: The study aimed to investigate the association between two common polymorphisms in the gene encoding TGF- β 1 and the risk for the development of ILD and whether those polymorphisms affect the level of TGF- β 1 or have a relation with disease prognosis.Methods: Case control study included 90 subjects who were classified into: 56 patients suffering from ILD and 34 healthy subjects served as controls. Serum and BAL TGFB1ss levels were determined by enzyme-linked immunosorbent assay. Genotyping for codon 10 and codon 25 polymorphisms by PCR-RFLP analysis was performed to be correlated with the serum and BAL levels of TGF β 1 and disease prognosis.Results: The allele frequencies of both polymorphisms showed no significant differences between patients and controls. However the CC genotype of codon 10 polymorphism showed significant correlation with higher levels of serum and BAL TGF- β 1 and worse prognosis of the disease.Conclusion: No significant association between codon 10 and codon 25 polymorphisms of TGF- β 1 and the risk for development of ILD while there was association between the genetic variants of codon 10 polymorphism and the level of TGF- β 1 and the prognosis of the disease. ER -