RT Journal Article
SR Electronic
T1 Hypertonicity increases apparent water permeability in benign mesothelial cells but differentially regulates it in epithelioid mesothelioma cells depending on the osmotic agent
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA4349
DO 10.1183/13993003.congress-2015.PA4349
VO 46
IS suppl 59
A1 Evgeniy Solenov
A1 Lubov Katkova
A1 Galina Baturina
A1 Vassiliki Peppa
A1 Chrissi Hatzoglou
A1 Konstantinos Gourgoulianis
A1 Sotirios Zarogiannis
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/PA4349.abstract
AB Background: Changes in water permeability of neoplastic cells could lead to enhanced cell survival, metastatis and relapse incidence and chemo resistance. The regulation of water permeability in normal mesothelial cells and in malignant pleural mesothelioma (MPM) cells has not been evaluated.Aims: Our aim was to assess the influence of hypertonic challenge on the apparent water permeability [Pf (cm/s)] in the normal immortalized mesothelial cell line Met5A and in the malignant epithelioid MPM cell line M14K.Methods: Cells were grown in RPMI culture medium. The cells underwent hypertonic challenge for 24 hours by adding 4% of glucose or mannitol. Pf was measured biophysically by the calcein quenching method. In all experimental conditions n>15.Results: In control conditions (isotonic) the Pf of Met5A was significantly higher than that of M14K, showing that malignant cells are less permeable to water (p<0.001). After the hypertonic stress the cells exhibited differences in the Pf. The Pf of Met5A cells increased significantly [Control 2.5±0.3; Mannitol 7.0±0.06; Glucose 7.3±1.0; (x10-3) cm/s; p<0.001 vs. control]. In M14K mannitol significantly decreased and glucose significantly increased the Pf [Control 1.43±0.12; Mannitol 0.88±0.06; Glucose 1.88±0.3 (x10-3) cm/s; p<0.001 vs. control].Conclusions: The Pf of Met5A is significantly higher than that of M14K cells. In hypertonic stress in Met5A cells the Pf significantly increases irrespective of the type of the osmotic agent. In MPM cells the type of osmotic agent differentially regulates the Pf. These results indicate a role for Pf in MPM pathobiology.