TY - JOUR T1 - Gene-Xpert for rapid detection of MDR-TB in re-treatment cases;a comparison with standard culture and DST JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA1526 VL - 46 IS - suppl 59 SP - PA1526 AU - Shahid Pervaiz AU - Muhammad Wajaht Tariq AU - Humayoun Ghulam Murtaza AU - Muhammad Zubair Shaheen Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA1526.abstract N2 - Objective: To evaluate the role of Gene-Xpert in early detection of MDR-TB in re-treatment cases and its comparison with standard culture and DSTMethod: This is a descriptive study.Location of the study is the Chest Clinic of Tertiary Care Hospital.We enrolled 133 patients over a period of 23 months who fulfilled eligibility criteria.Sputum for AFB smear and Gene-Xpert was conducted for MTB and Rifampicin resistance.All patients whose Gene-Xpert was positive for MTB,were undergone MTB culture and DST to determine Rifampicin/Multidrug resistance.Sensitivity and specificity of Gene-Xpert was calculated.Results: Of the total(N=133)patients,123(92.48%)were sputum AFB smear positive and 10(7.52%)were negative.All 133 were positive for MTB on Gene-Xpert.In sputum smear positive patients (123),Gene-Xpert MTB was positive in all 123 and Gene-Xpert Rifampicin resistance was positive in 113 (91.86%) and negative in 10 (8.13%).In 113 patients whose Rifampicin Resistance on Gene-Xpert was positive,112 (99.11%) were positive for Rifampicin resistance on DST and 1(.008%)was negative.In 10 patients who were Rifampicin resistance negative on Gene-Xpert,1 was Rifampicin resistance positive on DST and 9 were negative.In 10 cases who were sputum smear negative,Gene-Xpert MTB was positive in all 10 and all were Rifampicin resistance positive on Gene-Xpert and DST.Gene-Xpert Specificity was 90% and sensitivity 99.18%.Conclusion: Frequency of drug resistance is high in re-treatment cases of TB and using Gene-Xpert as a primary tool for early detection of Rifampicin resistance is an important clue for MDR-TB.It emphasis the need for routine use of Gene-Xpert,Culture and DST in re-treatment cases of TB. ER -