TY - JOUR T1 - Features of rat tracheal epithelium regeneration JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA3028 VL - 46 IS - suppl 59 SP - PA3028 AU - A. Volkova AU - E. Mubarakshina AU - A. Gordeeva AU - A. Temnov AU - I. Klimanov AU - S. Soodaeva AU - V. Novoselov Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA3028.abstract N2 - We studied the regeneration of rat respiratory epithelium following hydrochloric vapor inhalation injury. Activation of regeneration was carried out by application of paracrine factors of mesenchymal stem cells (MSC) into the trachea after chemical burn. The results, obtained by application MSC paracrine factors into the burnt trachea 30 minutes after the burn, completely differ. Morphological analysis of epithelium structure of trachea, taken 3 days after the injury and following application of MSC paracrine factors, has shown the considerable preservation of epithelium, there are ciliated cells in the epithelium that are lacking in test experiments after burns which indicates strong regeneration processes in the trachea when MSC paracrine factors applied. Upon application of MSC paracrine factors there were some closed-shaped formations of epithelial nature founded in the submucosal layer. The significant feature of these formations is that the internal surface of them is covered with ciliated cells, similar to epithelium ciliated cells. They appear on the first day after injury and MSC paracrine factors application, then their quantity increases and reach a maximum at the 3 or 4 day. Farther the number of formations decreases. The club cells were quite broadly presented there. Maximal quantity of the closed-shaped formations is observed at the 3-4 day after the burn and MSC paracrine factors application; there are not detected formations at the end of 7 day. The formations are found near the basement membrane, where they build into the respiratory epithelium and form the new section. The present study demonstrate the second way of epithelium regeneration, by means of epithelium formations building in the submucosal layer. ER -