RT Journal Article
SR Electronic
T1 Endothelial microparticles in sputum of COPD patients: A potential biomarker of disease
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3879
DO 10.1183/13993003.congress-2015.PA3879
VO 46
IS suppl 59
A1 Donato Lacedonia
A1 Grazia Pia Palladino
A1 Chiara Porro
A1 Giovanna E. Carpagnano
A1 Teresa Trotta
A1 Luigi D. Zoppo
A1 Valerio Saliani
A1 Maria Pia Foschino Barbaro
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/PA3879.abstract
AB Introduction: Microparticles (MPs) are small membrane vesicles of 0,1-1 µm released by cells, following chemical, physical and apoptotic stimuli. MPs represent more than just a miniature version of the cell, their composition and their function depend not only by cellular origin but also by stimuli. Chronic obstructive pulmonary diseases (COPD) is a lung disease characterized by nearly irreversible lung destruction which result in airway limitation.Aims: We investigated the presence and the source of MPs in sputum COPD patients to evaluate if changes in MPs number and composition may reflect the pathophysiological conditions of disease, and may serve as potential biomarkers for diagnostic and prognostic use.Methods: Induced sputum sample were collected from 18 male subjects and liquefied with sputasol. MPs obtained were analized by cytofluorimetry and immunolabelling for leukocyte (CD11a), granulocyte (CD66b), monocyte-macrophage (CD11b), platelets and megakaryocytic ( CD41), endothelial CD31, and from red blood cells (CD235ab).Results: There was a negative correlation between CD31-MPs and FEV1 (R=-53, p<0.05), CD66-MPs was correlated with worse performance index of COPD (BODE) and negative correlated with Walking Test: 0.65 and -0.64 respectively (p<0.05). CD235 showed a negative correlation with BMI (R=-0.86, p<0.05) while a positive correlation with dyspnoea index (R=0.91, p<0.05).Conclusion: MPs are detected in sputum of patients affected by COPD. The phenotype of some of them are related with main COPD parameters. These results suggest that MPs are likely implicated in the pathogenesis, or in evolution, of COPD.