TY - JOUR T1 - Endothelial microparticles in sputum of COPD patients: A potential biomarker of disease JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA3879 VL - 46 IS - suppl 59 SP - PA3879 AU - Donato Lacedonia AU - Grazia Pia Palladino AU - Chiara Porro AU - Giovanna E. Carpagnano AU - Teresa Trotta AU - Luigi D. Zoppo AU - Valerio Saliani AU - Maria Pia Foschino Barbaro Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA3879.abstract N2 - Introduction: Microparticles (MPs) are small membrane vesicles of 0,1-1 µm released by cells, following chemical, physical and apoptotic stimuli. MPs represent more than just a miniature version of the cell, their composition and their function depend not only by cellular origin but also by stimuli. Chronic obstructive pulmonary diseases (COPD) is a lung disease characterized by nearly irreversible lung destruction which result in airway limitation.Aims: We investigated the presence and the source of MPs in sputum COPD patients to evaluate if changes in MPs number and composition may reflect the pathophysiological conditions of disease, and may serve as potential biomarkers for diagnostic and prognostic use.Methods: Induced sputum sample were collected from 18 male subjects and liquefied with sputasol. MPs obtained were analized by cytofluorimetry and immunolabelling for leukocyte (CD11a), granulocyte (CD66b), monocyte-macrophage (CD11b), platelets and megakaryocytic ( CD41), endothelial CD31, and from red blood cells (CD235ab).Results: There was a negative correlation between CD31-MPs and FEV1 (R=-53, p<0.05), CD66-MPs was correlated with worse performance index of COPD (BODE) and negative correlated with Walking Test: 0.65 and -0.64 respectively (p<0.05). CD235 showed a negative correlation with BMI (R=-0.86, p<0.05) while a positive correlation with dyspnoea index (R=0.91, p<0.05).Conclusion: MPs are detected in sputum of patients affected by COPD. The phenotype of some of them are related with main COPD parameters. These results suggest that MPs are likely implicated in the pathogenesis, or in evolution, of COPD. ER -