TY - JOUR T1 - Monocyte-derived macrophages from non-smoker COPD subjects exhibit defective bacterial phagocytosis JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2015.PA380 VL - 46 IS - suppl 59 SP - PA380 AU - Baishakhi Ghosh AU - Kanchan Pyasi AU - Jyoti Londhe AU - Sushma Jadhav AU - Sapna Madas AU - Bill Brashier AU - Sundeep Salvi AU - Peter Barnes AU - Louise Donnelly Y1 - 2015/09/01 UR - http://erj.ersjournals.com/content/46/suppl_59/PA380.abstract N2 - Background: We have previously reported that monocytes-derived macrophages (MDM) from smoker COPD (S-COPD) subjects show defective bacterial phagocytosis. However, it is not known whether the same holds true for non-smoker COPD (NS-COPD) subjects.We aimed to study the phagocytic activity of MDM from NS-COPD subjects and compare it with S-COPD and healthy (H) subjects from India.Methods: Monocytes were derived from peripheral venous blood from 22 H subjects, 10 S-COPD subjects and 15 NS-COPD subjects (10 biomass fuel smoke exposed and 5 from other causes). The monocytes were cultured with complete media containing granulocyte-monocyte colony stimulating factor for 16-20 days to obtain MDM. 1X106 MDM/well were incubated with fluorescently-tagged, heat-killed S. pneumoniae (SP: 1.2X109 CFU/mL) and H. influenzae (HI: 1.5X108 CFU/mL) for 4 hours and fluorescence was measured to quantify the intracellular uptake of SP and HI.Results: Conclusion: Monocyte-derived macrophages of non-smoker COPD subjects show defective phagocytosis, which provides further evidence to suggest that there is a common susceptibility mechanism. ER -