RT Journal Article
SR Electronic
T1 Macrophage morphology in sputum samples from paediatric CF patients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA1302
DO 10.1183/13993003.congress-2015.PA1302
VO 46
IS suppl 59
A1 Rina Shah
A1 Abigail Whitehosue
A1 Naseem Mushtaq
A1 Jonathan Grigg
YR 2015
UL http://erj.ersjournals.com/content/46/suppl_59/PA1302.abstract
AB Introduction: Respiratory failure, secondary to persistent respiratory infections and chronic inflammation, is the leading cause of death in cystic fibrosis (CF). Studies show that samples from patients with CF contain a higher number of “small macrophages” when analysed through flow cytometry; resulting in lower phagocytic capabilities but higher inflammatory properties [1].Aims: We sought to explore whether morphological differences are found in sputum macrophages from paediatric CF patients.Methods: Sputum was collected from 5 CF patients and healthy controls (aged 7-16 years). Samples were processed and stained with a Hemacolor kit. Images of cells were taken at (x100) and morphology was analysed using Image J [2].Results: Total macrophage size was significantly smaller (p<0.005 **) in CF sputum (225.1 µm2 ± 41.85) vs controls (552.4 µm2 ± 57.18). Macrophage nuclei were also significantly smaller (p<0.05 *) in CF (64.59 µm2 ± 6.652 vs 96.13 µm2 ± 7.584). Figure 1. Conclusion: Cytospin findings are compatible with the conclusion that significant morphological changes occur in macrophages of paediatric CF patients in particular the visible presence of “small macrophages”. This information gives further insight into the function of macrophages within the diseased lung.1. Garratt, L.W., et al., Small macrophages are present in early childhood respiratory disease. J Cyst Fibros, 2012. 11(3): p. 201-8.2. Image J. Available from: http://imagej.nih.gov/ij/.