RT Journal Article
SR Electronic
T1 Pyrosequencing reveals transient cystic fibrosis lung microbiome changes with intravenous antibiotics
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP erj02030-2013
DO 10.1183/09031936.00203013
A1 Daniel J. Smith
A1 Alison C. Badrick
A1 Martha Zakrzewski
A1 Lutz Krause
A1 Scott C. Bell
A1 Gregory J. Anderson
A1 David W. Reid
YR 2014
UL http://erj.ersjournals.com/content/early/2014/07/17/09031936.00203013.abstract
AB Chronic airway infection in adults with cystic fibrosis (CF) is polymicrobial and the impact of intravenous antibiotics on the bacterial community composition is poorly understood. We employed culture-independent molecular techniques to explore the early effects of i.v. antibiotics on the CF airway microbiome. DNA was extracted from sputum samples collected from adult subjects with CF at three time-points (before starting treatment, and at day 3 and day 8–10 of i.v. antibiotics) during treatment of an infective pulmonary exacerbation. Microbial community profiles were derived through analysis of bacterial-derived 16S ribosomal RNA by pyrosequencing and changes over time were compared. 59 sputum samples were collected during 24 pulmonary exacerbations from 23 subjects. Between treatment onset and day 3 there was a significant reduction in the relative abundance of Pseudomonas and increased microbial diversity. By day 8–10, bacterial community composition was similar to pre-treatment. Changes in community composition did not predict improvements in lung function. The relative abundance of Pseudomonas falls rapidly in subjects with CF receiving i.v. antibiotic treatment for a pulmonary exacerbation and is accompanied by an increase in overall microbial diversity. However, this effect is not maintained beyond the first week of treatment. Changes in the CF microbiome in response to i.v. antibiotics are not sustained despite ongoing antibiotic pressure http://ow.ly/wzYyg