RT Journal Article
SR Electronic
T1 Breath analysis during one-lung ventilation in cancer patients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP erj01254-2011
DO 10.1183/09031936.00125411
A1 S. Kischkel
A1 W. Miekisch
A1 P. Fuchs
A1 J.K. Schubert
YR 2012
UL http://erj.ersjournals.com/content/early/2012/01/19/09031936.00125411.abstract
AB Non-invasive breath analysis may provide valuable information for cancer recognition if disease specific volatile biomarkers could be identified. In order to compare non-diseased and diseased tissue in vivo this study took advantage of the special circumstances of one-lung ventilation (OLV) during lung-surgery.15 cancer patients undergoing lung resection with OLV were enrolled. From each patient alveolar breath samples were taken separately from healthy and diseased lungs before and after tumour resection. Volatile substances were preconcentrated by means of solid-phase microextraction, separated, identified and quantified by means of GC-MS.Different classes of volatile substances could be identified according to their concentration profiles. Due to prolonged fasting and activation of lipolysis concentrations of endogenous acetone significantly increased during surgery. Exogenous substances such as benzene or cyclohexanone showed typical washout exhalation kinetics. Exhaled concentrations of potentially tumour associated substances such as butane or pentane were different for non-diseased and diseased lungs and decreased significantly after surgery.Separate analysis of volatile substances exhaled from healthy and diseased lungs in the same patient, together with thorough consideration of substance origins and exhalation kinetics offers unique opportunities of biomarker recognition and evaluation.