RT Journal Article
SR Electronic
T1 Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP erj01532-2011
DO 10.1183/09031936.00153211
A1 Chimenti, Laura
A1 Luque, Tomás
A1 Bonsignore, Maria R.
A1 Ramírez, José
A1 Navajas, Daniel
A1 Farré, Ramon
YR 2012
UL http://erj.ersjournals.com/content/early/2012/03/22/09031936.00153211.abstract
AB Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator-induced lung injury (VILI).Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation.24 Sprague-Dawley rats (250–300 g) were subjected to high-volume mechanical ventilation (25 mL·kg−1). MSCs (5×106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis.When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung oedema , histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally.Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.