Supplementary figures

Supplementary figures

Files in this Data Supplement:

  • Supplementary figure 1 - Spn infection concurrent and after, but not before (resolved), sensitisation induces CD4+CD25+Foxp3+ Tregs in AAD. (A) Flow cytometry histogram showing the infection concurrent with sensitisation increases Tregs in MLNs. (B) Total numbers, (C) percentages and (D) histograms of CD4+CD25+Foxp3+ Treg cells in MLNs with infection after or before (resolved) sensitisation. # and * represent significant differences compared to uninfected, nonallergic (Saline) and uninfected, allergic (OVA) controls, respectively. #/* p <_0.05.-- end="end" desc="desc" supplementary_figure_1.jpg="supplementary_figure_1.jpg" _--="_--">
  • Supplementary figure 2 - Anti-CD25 treatment reverses the suppressive systemic effects of Spn infection on AAD. OVA-induced cytokine release in splenocyte culture supernatants. # and * represent significant differences compared to uninfected, nonallergic (Saline) and uninfected, allergic (OVA) controls, respectively. #/* p <_0.05 _="_" p0.01="p0.01" and="and" p0.001.--="p0.001.--" end="end" desc="desc" supplementary_figure_2.jpg="supplementary_figure_2.jpg" _--="_--">
  • Supplementary figure 3 - Isotype control antibody does not alter the suppressive effects of Spn infection on AAD. OVA-induced cytokine release in (A) MLN and (B) splenocyte culture supernatants. (C) OVA-specific IgG1 serum antibodies. (D) Eosinophil accumulation in blood, BALF and tissue. (E) Mucus secreting cells surrounding the airway lumen. AHR in terms of averaged peak (F) transpulmonary resistance, and (G) dynamic compliance in response to increasing doses of methacholine; N.B. significant differences in resistance and compliance are for the entire dose response curves. # and * represent significant differences compared to uninfected, nonallergic (Saline) and uninfected, allergic (OVA) controls, respectively. #/* p <_0.05 _="_" p0.01="p0.01" and="and" p0.001="p0.001" n.d.="n.d." no="no" significant="significant" difference.--="difference.--" end="end" desc="desc" supplementary_figure_3.jpg="supplementary_figure_3.jpg" _--="_--">