Supplementary methods and results

Supplementary methods and results

Files in this Data Supplement:

• Supplementary methods and results - Methods
  Airway neutrophil isolation
  Peripheral blood neutrophil isolation
  Neutrophil culture and stimulation
  Results
  Table E1: Clinical characteristics of participants with eosinophilic and non-eosinophilic asthma selected for microarray analysis
  Table E2: Relative levels of cytokine gene expression in resting and LPS stimulated peripheral blood neutrophils isolated from participants with eosinophilic (n=8) and non-eosinophilic asthma (n=9) and healthy controls (n=11)
  Table E3: Mean fold changes in gene expression of selected targets identified to be differentially expressed by microarrays and confirmed by relative real-time PCR
  Table E4: Spearman Rank Correlation Analysis between the expression of evaluated up-regulated genes in noneosinophilic asthma and lung function (FEV1% predicted; n=9)