Supplementary methods and figures

Supplementary methods and figures

Files in this Data Supplement:

• Supplementary methods and figures - Methods: histochemistry; RNA extraction and real-time quantitative PCR; protein extraction; gel electrophoresis and immunoblotting; NF-κB activity; Figure E1. Representative blots stained with PonceauS, illustrating equal loading for MyoD protein (A), myogenin protein (B) and Myostatin nuclear protein (C) in the diaphragm of 6 different controls and 6 different COPD patients. Figure E2. Western blot illustrating Histone-H3 (16 kDa) expression in the diaphragm of 6 different controls and 6 different COPD patients.