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Supplementary material

Supplementary material

Supplementary figures, tables and videos

Files in this Data Supplement:

  • Supplementary figures -
    Figure S1. M2R-immunoreactivity in M2R-/- animals. Triple-labeling immunohistochemistry shows that M2R-immunoreactivity was absent from the smooth muscle cells (labeled with α-smooth muscle cell actin, B) of the m. trachealis (m) but labeling was still present in pericyte-like cells surrounding CD31-immunoreactive endothelial cells (C) in the lamina propria (arrows). (D) Merge of A-C. Bar = 50 μm.
    Figure S2. Rüdeberg-stained semi-thin sections of the tracheae of M1R-/-, M2R-/-, and M3R-/- and their corresponding WT mice in regions overlying cartilage, muscle and ligament, respectively. Bar = 20 μm.
    Figure S3. Immunoreactivity for villin (A-D) and PGP 9.5 (E-H) in the tracheal epithelium of M1R-/-, M2R-/-, and M3R-/- mice and their WT controls. Bar = 10 μm
  • Supplementary tables -
    Table S1. Proportion of ciliated, non-ciliated, and basal cells in the epithelium of the cervical and thoracic trachea.
    Table S2. Number of villin- and PGP 9.5-immunoreactive cells per trachea section.
    Table S3. Primer pairs for real-time RT-PCR
  • Supplementary movie 1
  • Supplementary movie 2

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