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Single cell RNA Sequencing Identifies G-protein Coupled Receptor 87 as a Basal Cell Marker Expressed in Distal Honeycomb Cysts in Idiopathic Pulmonary Fibrosis

Katharina Heinzelmann, Qianjiang Hu, Yan Hu, Evgenia Dobrinskikh, Meshal Ansari, M Camila Melo-Narváez, Henrik M. Ulke, Colton Leavitt, Carol Mirita, Tammy Trudeau, Maxwell L. Saal, Pamela Rice, Bifeng Gao, William J. Janssen, Ivana V. Yang, Herbert B. Schiller, Eszter K. Vladar, Mareike Lehmann, Melanie Königshoff
European Respiratory Journal 2022; DOI: 10.1183/13993003.02373-2021
Katharina Heinzelmann
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
7Authors contributed equally
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Qianjiang Hu
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
6Division of Pulmonary, Allergy and Critical Care Medicine, School of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
7Authors contributed equally
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  • ORCID record for Qianjiang Hu
Yan Hu
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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Evgenia Dobrinskikh
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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Meshal Ansari
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
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M Camila Melo-Narváez
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
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Henrik M. Ulke
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
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Colton Leavitt
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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Carol Mirita
3Eastern Colorado VA Healthcare System, Rocky Mountain Regional VA Medical Center, Aurora, CO, USA
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Tammy Trudeau
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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Maxwell L. Saal
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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Pamela Rice
3Eastern Colorado VA Healthcare System, Rocky Mountain Regional VA Medical Center, Aurora, CO, USA
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Bifeng Gao
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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William J. Janssen
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
4Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, National Jewish Health, Denver, CO, USA
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Ivana V. Yang
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
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Herbert B. Schiller
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
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Eszter K. Vladar
2Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO, USA
5Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO, USA
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Mareike Lehmann
1Institute of Lung Health and Immunity, Comprehensive Pneumology Center Munich, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
8Authors contributed equally, co-corresponding authors
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Melanie Königshoff
6Division of Pulmonary, Allergy and Critical Care Medicine, School of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
8Authors contributed equally, co-corresponding authors
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  • For correspondence: koenigshoffm@upmc.edu
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Footnotes

This manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.

Conflicts of interest: All authors declare no conflicts of interest.

This is a PDF-only article. Please click on the PDF link above to read it.

  • Received August 30, 2021.
  • Accepted March 2, 2022.
  • Copyright ©The authors 2022.
http://creativecommons.org/licenses/by-nc/4.0/

This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. For commercial reproduction rights and permissions contact permissions{at}ersnet.org

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Single cell RNA Sequencing Identifies G-protein Coupled Receptor 87 as a Basal Cell Marker Expressed in Distal Honeycomb Cysts in Idiopathic Pulmonary Fibrosis
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Single cell RNA Sequencing Identifies G-protein Coupled Receptor 87 as a Basal Cell Marker Expressed in Distal Honeycomb Cysts in Idiopathic Pulmonary Fibrosis
Katharina Heinzelmann, Qianjiang Hu, Yan Hu, Evgenia Dobrinskikh, Meshal Ansari, M Camila Melo-Narváez, Henrik M. Ulke, Colton Leavitt, Carol Mirita, Tammy Trudeau, Maxwell L. Saal, Pamela Rice, Bifeng Gao, William J. Janssen, Ivana V. Yang, Herbert B. Schiller, Eszter K. Vladar, Mareike Lehmann, Melanie Königshoff
European Respiratory Journal Jan 2022, 2102373; DOI: 10.1183/13993003.02373-2021

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Single cell RNA Sequencing Identifies G-protein Coupled Receptor 87 as a Basal Cell Marker Expressed in Distal Honeycomb Cysts in Idiopathic Pulmonary Fibrosis
Katharina Heinzelmann, Qianjiang Hu, Yan Hu, Evgenia Dobrinskikh, Meshal Ansari, M Camila Melo-Narváez, Henrik M. Ulke, Colton Leavitt, Carol Mirita, Tammy Trudeau, Maxwell L. Saal, Pamela Rice, Bifeng Gao, William J. Janssen, Ivana V. Yang, Herbert B. Schiller, Eszter K. Vladar, Mareike Lehmann, Melanie Königshoff
European Respiratory Journal Jan 2022, 2102373; DOI: 10.1183/13993003.02373-2021
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