Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Subscriptions

Nasopharyngeal metatranscriptome profiles of infants with bronchiolitis and risk of childhood asthma: a multicentre prospective study

Yoshihiko Raita, Marcos Pérez-Losada, Robert J. Freishtat, Andrea Hahn, Eduardo Castro-Nallar, Ignacio Ramos-Tapia, Nathaniel Stearrett, Yury A. Bochkov, James E. Gern, Jonathan M. Mansbach, Zhaozhong Zhu, Carlos A. Camargo, Kohei Hasegawa
European Respiratory Journal 2021; DOI: 10.1183/13993003.02293-2021
Yoshihiko Raita
1Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yoshihiko Raita
  • For correspondence: yraita1@alumni.jh.edu
Marcos Pérez-Losada
2Department of Biostatistics and Bioinformatics, Computational Biology Institute, The George Washington University, Washington, DC, USA
3CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert J. Freishtat
4Center for Genetic Medicine Research, Children's National Research Institute, Washington, DC, USA
5Division of Emergency Medicine, Children's National Hospital, Washington, DC, USA
6Department of Pediatrics, George Washington University School of Medicine and Health Sciences, Washington, DC, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrea Hahn
4Center for Genetic Medicine Research, Children's National Research Institute, Washington, DC, USA
6Department of Pediatrics, George Washington University School of Medicine and Health Sciences, Washington, DC, USA
7Division of Infectious Diseases, Children's National Hospital, Washington, DC, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Andrea Hahn
Eduardo Castro-Nallar
8Centro de Bioinformática y Biología Integrativa, Universidad Andres Bello, Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ignacio Ramos-Tapia
8Centro de Bioinformática y Biología Integrativa, Universidad Andres Bello, Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathaniel Stearrett
9Computational Biology Institute, George Washington University, Washington, DC, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yury A. Bochkov
10Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James E. Gern
10Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA
11Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for James E. Gern
Jonathan M. Mansbach
12Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhaozhong Zhu
1Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Zhaozhong Zhu
Carlos A. Camargo
1Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carlos A. Camargo
Kohei Hasegawa
1Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

The question addressed by the study Bronchiolitis is not only the leading cause of hospitalisation in U.S. infants but also a major risk factor for asthma development. Growing evidence supports clinical heterogeneity within bronchiolitis. To identify metatranscriptome profiles of infant bronchiolitis, and examine their relationship with host transcriptome and subsequent asthma development.

Materials/patients and methods As part of multicentre prospective cohort study of infants (age <12 months) hospitalised for bronchiolitis, we integrated virus and nasopharyngeal metatranscriptome (species-level taxonomy and function) data measured at hospitalisation. We applied network-based clustering approaches to identify metatranscriptome profiles. We then examined their association with host transcriptome at hospitalisation and risk for developing asthma.

Results We identified five metatranscriptome profiles of bronchiolitis (n=244):

A) virusRSVmicrobiomecommensals, B) virusRSV/RV-AmicrobiomeH.influenzae,

C) virusRSVmicrobiomeS.pneumoniae, D) virusRSVmicrobiomeM.nonliquefaciens, and

E) virusRSV/RV-CmicrobiomeM.catarrhalis. Compared with profile A, profile B infants were characterised by high proportion of eczema, H. influenzae abundance, and enriched virulence related to antibiotic resistance. These profile B infants also had upregulated TH17 and downregulated type I interferon pathways (FDR<0.005) and significantly higher risk for developing asthma (17.9% versus 38.9%; adjOR, 2.81; 95%CI, 1.11–7.26). Likewise, profile C infants were characterised by high proportion of parental asthma, S. pneumoniae dominance, and enriched glycerolipid and glycerophospholipid metabolism of microbiome. These profile C infants had upregulated receptor for advanced glycation end products signalling pathway (FDR<0.005) and higher risk of asthma (17.9% versus 35.6%; adjOR, 2.49; 95%CI, 1.10–5.87).

Answer to the question Metatranscriptome and clustering analysis identified biologically-distinct metatranscriptome profiles that have differential risks of asthma.

Footnotes

This manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.

Conflict of interest: Yoshihiko Raita has nothing to disclose.

Conflict of interest: Marcos Pérez-Losada has nothing to disclose.

Conflict of interest: Robert J. Freishtat has nothing to disclose.

Conflict of interest: Andrea Hahn has nothing to disclose.

Conflict of interest: Eduardo Castro-Nallar has nothing to disclose.

Conflict of interest: Ignacio Ramos-Tapia has nothing to disclose.

Conflict of interest: Nathaniel Stearrett has nothing to disclose.

Conflict of interest: Dr. Yury A. Bochkov has patents on production methods of rhinoviruses.

Conflict of interest: Dr. James E. Gern is a paid consultant to AstraZeneca and Meissa Vaccines Inc., has stock options in Meissa Vaccines Inc., and has patents on production methods of rhinoviruses.

Conflict of interest: Jonathan M. Mansbach has nothing to disclose.

Conflict of interest: Zhaozhong Zhu has nothing to disclose.

Conflict of interest: Carlos A. Camargo Jr. has nothing to disclose.

Conflict of interest: Kohei Hasegawa has nothing to disclose.

  • Received August 20, 2021.
  • Accepted November 17, 2021.
  • Copyright ©The authors 2021. For reproduction rights and permissions contact permissions{at}ersnet.org
View Full Text

ERS Members

myERS - ERS members : log in with your myERS username and password.

INDIVIDUALS

Log in Login as an individual user.

Forgot your username or password?

LIBRARY USERS

Log in through your institution

If your library has a subscription, you may already be logged in via your IP address. Otherwise you may be able to log in via one of the following routes.
You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.
If you think you should have access, please contact your librarian or email journals@ersnet.org

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top
View this article with LENS
Vol 61 Issue 2 Table of Contents
European Respiratory Journal: 61 (2)
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Nasopharyngeal metatranscriptome profiles of infants with bronchiolitis and risk of childhood asthma: a multicentre prospective study
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Citation Tools
Nasopharyngeal metatranscriptome profiles of infants with bronchiolitis and risk of childhood asthma: a multicentre prospective study
Yoshihiko Raita, Marcos Pérez-Losada, Robert J. Freishtat, Andrea Hahn, Eduardo Castro-Nallar, Ignacio Ramos-Tapia, Nathaniel Stearrett, Yury A. Bochkov, James E. Gern, Jonathan M. Mansbach, Zhaozhong Zhu, Carlos A. Camargo, Kohei Hasegawa
European Respiratory Journal Jan 2021, 2102293; DOI: 10.1183/13993003.02293-2021

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Nasopharyngeal metatranscriptome profiles of infants with bronchiolitis and risk of childhood asthma: a multicentre prospective study
Yoshihiko Raita, Marcos Pérez-Losada, Robert J. Freishtat, Andrea Hahn, Eduardo Castro-Nallar, Ignacio Ramos-Tapia, Nathaniel Stearrett, Yury A. Bochkov, James E. Gern, Jonathan M. Mansbach, Zhaozhong Zhu, Carlos A. Camargo, Kohei Hasegawa
European Respiratory Journal Jan 2021, 2102293; DOI: 10.1183/13993003.02293-2021
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
    • Abstract
  • Info & Metrics
  • PDF

Subjects

  • Paediatric pulmonology
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Early-life and health behaviour influences on lung function in early-adulthood
  • Respiratory recovery trajectories after severe-to-critical COVID-19: a 1-year prospective multicentre study
  • Inhibition of MRTF activation as a clinically achievable anti-fibrotic mechanism for Pirfenidone
Show more Original research article

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society